Characterization of virus-like particles associated with the human faecal and caecal microbiota

This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such s...

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Published inResearch in microbiology Vol. 165; no. 10; pp. 803 - 812
Main Authors Hoyles, Lesley, McCartney, Anne L., Neve, Horst, Gibson, Glenn R., Sanderson, Jeremy D., Heller, Knut J., van Sinderen, Douwe
Format Journal Article
LanguageEnglish
Published France Elsevier Masson SAS 01.12.2014
Subjects
Adult
Bacteriophages
Biodiversity
Caecum
Cecum - virology
Faeces
Feces - virology
Female
Gastrointestinal tract
Humans
Male
Microbial ecology
Microbiota
Middle Aged
Transmission electron microscopy
Viruses - classification
Viruses - genetics
Viruses - isolation & purification
Young Adult
Bacteriophages
Microbial ecology
Gastrointestinal tract
Transmission electron microscopy
Faeces
Caecum
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Summary:This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0923-2508
1769-7123
DOI:10.1016/j.resmic.2014.10.006