Palatal microbiota associated with membranous substances in older Japanese individuals undergoing tube feeding in long-term care: A cross-sectional study

• Published in: Heliyon Vol. 9; no. 10; p. e20401
• Main Authors: Asahina, Hironao, Ogasawara, Tadashi, Akieda, Toshie, Miyahara, Kohta, Okada, Yoshiyuki, Matsumura, Kohei, Taniguchi, Makoto, Yoshida, Akihiro, Kakinoki, Yasuaki
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2023; Elsevier
• Subjects: Dental care for aged; Microbiota; Oral hygiene; Tube feeding; Xerostomia; Microbiota; Xerostomia; Oral hygiene; Dental care for aged; Tube feeding
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2023.e20401

Tube feeders are prone to membranous substance formation on the palate, and those with membranous substances have a risk of fever, with the probable involvement of their oral bacteria. However, the palatal microbiota of those with membranous substances has not been elucidated. Therefore, we evaluated the differences in palatal microbiota between tube-fed individuals with and without membranous substances to clarify the microbiota. This study included 19 participants aged 65 years who required tube feeding. The participants’ characteristics were collected from nursing records and oral examinations. If membranous materials were found on the palate, a specimen was collected. Membranous substances were defined as keratotic degeneration observed under a microscope. Additionally, we performed a comprehensive microbiome analysis by extracting DNA from the samples and performing 16 S rRNA gene sequencing. Finally, we compared the participant demographics and oral microbiota between patients with and without membranous substances. A total of 11 participants had membranous substances associated with “mouth dryness” (p < 0.001) and “constant mouth opening” (p = 0.020). Palatal microbiota differed between those with and without membranous substances. Among the bacteria with a relative abundance greater than 1.0%, the abundance of Streptococcus (p = 0.007), Fusobacterium (p = 0.041), Streptococcus agalactiae (p = 0.009), and Fusobacterium nucleatum subsp. vincentii (p = 0.026) was significantly higher in the membranous substance group than in the non-membranous substance group. The palatal microbiota of individuals undergoing tube feeding differed depending on the presence or absence of membranous substances. Membrane substance formation associated with dry mouth purportedly alters the palatal microbiota. Streptococcus, Fusobacterium, S. agalactiae, and F. nucleatum subsp. vincentii were more abundant in the oral microbiota of patients with membranous substances. Thus, preventing this formation may help in controlling the growth of these microbes. •Tube feeders are prone to membranous substance formation on the palate.•Unlike phlegm and crusts, membranous substances are related to fever in tube feeders.•Membranous substances are associated with mouth dryness and constant mouth opening.•Palatal microbiota differed between those with and those without membranous substances.•Preventing the formation of membranous substance may control unfavorable microbiota.