BYPASS1-LIKE, A DUF793 Family Protein, Participates in Freezing Tolerance via the CBF Pathway in Arabidopsis
The C-REPEAT BINDING FACTOR signaling pathway is strictly modulated by numerous factors and is essential in the cold response of plants. Here, we show that the DUF793 family gene modulates freezing tolerance through the CBFs in . The expression of was rapidly induced under cold treatment. Comparing...
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Published in | Frontiers in plant science Vol. 10; p. 807 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
26.06.2019
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Subjects | |
Online Access | Get full text |
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Summary: | The C-REPEAT BINDING FACTOR signaling pathway is strictly modulated by numerous factors and is essential in the cold response of plants. Here, we show that the DUF793 family gene
modulates freezing tolerance through the CBFs in
. The expression of
was rapidly induced under cold treatment. Comparing to wild type,
knockout mutants were more sensitive to freezing treatment, whereas B1L-overexpressing lines were more tolerant. The expression of
s and CBF target genes was significantly decreased in
mutant. Using yeast two-hybrid screening system, 14-3-3λ was identified as one of proteins interacting with B1L. The interaction was confirmed with bimolecular fluorescence complementation assay and co-immunoprecipitation assay. Biochemical assays revealed that
mutation promoted the degradation of CBF3 compared to wild type, whereas
mutant and
mutant suppressed the degradation of CBF3. Consistently,
and
mutants showed enhanced freezing tolerance compared to wild type. These results indicate that B1L enhances the freezing tolerance of plants, at least partly through stabilizing CBF. Our findings improve our understanding of the regulation of CBF in response to cold stress. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: William Walter Adams III, University of Colorado Boulder, United States Reviewed by: Jian-Kang Zhu, Purdue University, United States; Timothy Artlip, Appalachian Fruit Research Station (ARS-USDA), United States This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2019.00807 |