Development of enhanced selective media for detection of Vibrio parahaemolyticus in oysters

• Published in: Food science and biotechnology Vol. 30; no. 3; pp. 475 - 485
• Main Authors: Yoon, Jae-Hyun, Bae, Young-Min, Song, Hana, Lee, Soyul, Moon, Sung-Kwon, Oh, Se-Wook, Lee, Sun-Young
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.03.2021; Springer Nature B.V; 한국식품과학회
• Subjects: Bacteria; Bile salts; Chemistry; Chemistry and Materials Science; Citric acid; Food Science; Indicators; Nutrition; Oysters; pH effects; Research Article; Salts; Seafood; Selective media; Selectivity; Sodium chloride; Sodium citrate; Sucrose; Vibrio parahaemolyticus; 식품과학; Selective medium; Seafood; Food safety; Detection; Vibrio parahaemolyticus
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-021-00877-0

This study was undertaken to develop enhanced selective media for detection of Vibrio parahaemolyticus in oysters. Primarily, tryptic soy agar (TSA) was supplemented with 4.5–5% NaCl, 0.1–0.5% oxgall, and/or 1–2% sodium citrate, and adjusted to pH 8–9. A total of 21 Vibrio spp., 24 indicators, and 26 food-borne isolates were streaked on the modified media, followed by 24 h of incubation at 37 °C. While all the indicators and isolates failed to grow on TSA containing 5% NaCl, 0.5% oxgall, and 2% sodium citrate (TSA OSS1 ; pH 9), V. parahaemolyticus was culturable on this selective medium. Particularly, the ability of TSA OSS1 to quantify V . parahaemolyticus in oysters was superior to thiosulphate citrate bile salts sucrose (TCBS) agar. V. parahaemolyticus distinctly produced its white-yellowish, round, and edge-pointed colony on TSA OSS1 . TSAO SS1 with high selectivity potentials over TCBS may be a promising alternative for detection of V . parahaemolyticus in seafoods or natural reservoirs.