The Tumor Suppressor Role of Zinc Finger Protein 671 ( ZNF671 ) in Multiple Tumors Based on Cancer Single-Cell Sequencing

• Published in: Frontiers in oncology Vol. 9; p. 1214
• Main Authors: Zhang, Jian, Luo, Jianli, Jiang, Huali, Xie, Tao, Zheng, Jieling, Tian, Yunhong, Li, Rong, Wang, Baiyao, Lin, Jie, Xu, Anan, Huang, Xiaoting, Yuan, Yawei
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 08.11.2019
• Subjects: data mining; Oncology; single-cell sequencing; solid tumor; tumor suppressor; ZNF671; solid tumor; ZNF671; single-cell sequencing; tumor suppressor; data mining
• ISSN: 2234-943X; 2234-943X
• DOI: 10.3389/fonc.2019.01214

In humans, zinc finger protein 671 ( ) is a type of transcription factor. However, the contribution of tumor heterogeneity to the functional role of remains unknown. The present study aimed to determine the functional states of in cancer single cells based on single-cell sequencing datasets (scRNA-seq). We collected cancer-related scRNA-seq datasets and analyzed in the datasets. We evaluated 14 functional states of in cancers and performed expression and function state correlation analysis. We further applied t-distributed stochastic neighbor embedding to describe the distribution of cancer cells and to explore the functional state of in cancer subgroups. We found that was downregulated in eight cancer-related scRNA-seq datasets. Functional analysis identified that might play a tumor suppressor role in cancer. The heterogeneous functional states of cell subgroups and correlation analysis showed that played tumor suppressor roles in heterogeneous cancer cell populations. Western blot and transwell assays identified that inhibited EMT, migration, and invasion of CNS cancers, lung cancer, melanoma, and breast carcinoma . These results from cancer single-cell sequencing indicated that played a tumor suppressor role in multiple tumors and may provide us with new insights into the role of for cancer treatment.