Effect of granulocyte colony-stimulating factor on bone metabolism during peripheral blood stem cell mobilization

• Published in: International journal of hematology Vol. 77; no. 1; pp. 75 - 81
• Main Authors: WATANABE, Tsutomu, SUZUYA, Hiroko, TALMADGE, James E, ONISHI, Toshihiro, KANAI, Sachiyo, KANEKO, Michiya, WATANABE, Hiroyoshi, NAKAGAWA, Ryuji, KAWANO, Yoshifumi, TAKAUE, Yoichi, KURODA, Yasuhiro
• Format: Journal Article
• Language: English
• Published: Tokyo Springer 2003; Springer Nature B.V
• Subjects: Adolescent; Adult; Alkaline Phosphatase - blood; Amino Acids - blood; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; Biomarkers - blood; Blood. Blood coagulation. Reticuloendothelial system; Bone marrow, stem cells transplantation. Graft versus host reaction; Bone Remodeling - drug effects; Child; Child, Preschool; Cytokines - blood; Granulocyte Colony-Stimulating Factor - pharmacology; Hematopoietic Stem Cell Mobilization; Humans; Medical sciences; Osteocalcin - blood; Peripheral Blood Stem Cell Transplantation; Pharmacology. Drug treatments; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Human; Urine; Alkaline phosphatase; Hematology; Enzyme; Stem cell; Cytokine; Phosphoric monoester hydrolases; Hematopoietic cell; Biological marker; Esterases; Metabolism; Bone metabolism; G-CSF; Biological activity; Blood; Mobilization; Granulocyte colony stimulating factor; Polypeptide; Hydrolases; Serum; Bone; Peripheral blood stem cells
• ISSN: 0925-5710; 1865-3774
• DOI: 10.1007/bf02982606

Granulocyte colony-stimulating factor (G-CSF) has been shown to affect the biochemical markers of bone metabolism, including serum bone alkaline phosphatase (BALP), serum osteocalcin, and urine deoxypyridinoline. To determine the association between bone resorption and formation and the G-CSF-induced mobilization of peripheral blood stem cells (PBSC), we examined these markers during mobilization in 19 healthy donors. The average (+/- SEM) serum BALP level before treatment was 81.6 +/- 17.0 IU/dL, and the level increased significantly to 117.7 +/- 15.8 IU/dL on day 5 of G-CSF administration (P < .0001). The urine deoxypyridinoline level before treatment was 12.3 +/- 2.4 nmol/mmol creatinine, and this level also increased significantly to 19.4 +/- 3.0 nmol/mmol creatinine on day 5 of G-CSF administration (P < .0001). In contrast, the average level of serum osteocalcin significantly decreased from 8.07 +/- 2.88 ng/mL to 1.53 +/- 0.18 ng/mL on day 5 (P = .0353). During G-CSF administration, we also studied the serum levels of various cytokines (IL-1beta, osteoclastogenesis inhibitory factor [OCIF], IL-6, tumor necrosis factor alpha, transforming growth factor beta, interferon-gamma, macrophage colony-stimulating factor) related to bone metabolism. Only the kinetics of OCIF were significantly affected. The serum level of OCIF increased immediately after the start of G-CSF administration and remained high during G-CSF administration. These results demonstrate that high-dose G-CSF affects bone metabolism and that OCIF may play a role in bone metabolism. Consistent with the notion that G-CSF affects bone metabolism, a significant correlation was observed between CD34+ cell yield and the increase in urine deoxypyridinoline but not for the changes in serum BALP and osteocalcin levels. This result suggests that bone resorption is either directly or indirectly related to the mobilization of PBSC by G-CSF.