Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 17; no. 1; pp. 97 - 102
• Main Authors: Chung, Hyo-jin, Chung, Wook-hun, Lee, Jae-Hoon, Chung, Dai-Jung, Yang, Wo-Jong, Lee, A-Jin, Choi, Chi-Bong, Chang, Hwa-Seok, Kim, Dae-Hyun, Suh, Hyun Jung, Lee, Dong-Hun, Hwang, Soo-Han, Do, Sun Hee, Kim, Hwi-Yool
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한수의학회 01.03.2016; The Korean Society of Veterinary Science
• Subjects: Animals; Brain-Derived Neurotrophic Factor - genetics; Cord Blood Stem Cell Transplantation; Enzyme-Linked Immunosorbent Assay; Gene Expression Profiling; Gene Expression Regulation; Humans; Locomotion; Nerve Growth Factor - genetics; Original; Rats; Spinal Cord Injuries - therapy; 수의학; spinal cord injury; brain-derived neurotrophic factor; nerve growth factor; neurotrophic factor; neurotrophin-3
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2016.17.1.97

We induced percutaneous spinal cord injuries (SCI) using a balloon catheter in 45 rats and transplanted human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) at the injury site. Locomotor function was significantly improved in hUCB-MSCs transplanted groups. Quantitative ELISA of extract from entire injured spinal cord showed increased expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3). Our results show that treatment of SCI with hUCB-MSCs can improve locomotor functions, and suggest that increased levels of BDNF, NGF and NT-3 in the injured spinal cord were the main therapeutic effect.