Adult acute lymphoblastic leukemia with a rare b3a3 type BCR/ABL1 fusion transcript

• Published in: Cancer genetics Vol. 209; no. 4; pp. 161 - 165
• Main Authors: Kurita, Daisuke, Hatta, Yoshihiro, Hojo, Atsuko, Kura, Yoshimasa, Sawada, Umihiko, Kanda, Yoshinobu, Takei, Masami
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2016
• Subjects: Acute lymphoblastic leukemia; Adult; BCR/ABL1 fusion transcripts; Cytogenetic Analysis - methods; Fusion Proteins, bcr-abl - genetics; Hematology, Oncology and Palliative Medicine; Humans; In Situ Hybridization, Fluorescence - methods; Male; Medical Education; Philadelphia Chromosome; Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics; Acute lymphoblastic leukemia; BCR/ABL1 fusion transcripts; Philadelphia chromosome
• ISSN: 2210-7762; 2210-7770
• DOI: 10.1016/j.cancergen.2015.12.014

The Philadelphia chromosome (Ph) is the most frequent chromosomal abnormality detected in adult acute lymphoblastic leukemia (ALL). This chromosome forms the BCR/ABL1 fusion gene; thus, ABL1 exon a2 is generally used as a primer-binding region for the detection of the fusion transcript via reverse transcription polymerase chain reaction (RT-PCR). We observed a rare case of adult Ph-positive (Ph+ ) ALL, in which the BCR/ABL1 fusion transcript was not detected using the ABL1 exon a2 region primer. However, we were able to isolate a PCR product by RT-PCR with the BCR exon 13 (b2) and ABL1 exon a3 primers. Analysis of the sequence of the RT-PCR product revealed that the fusion point was between BCR exon 14 (b3) and ABL1 exon a3, and that the transcript lacked ABL1 exon a2. The patient achieved cytogenetic remission through combination chemotherapies, but relapse occurred before hematopoietic stem cell transplantation and the patient died 11 months after the initialization of chemotherapies. If the BCR/ABL1 fusion transcript is undetected with the ABL1 exon a2 region primer in Ph+ ALL cases, an RT-PCR analysis that can detect the b3a3 type BCR/ABL1 fusion transcript should be considered to improve diagnosis.