Genetic Characterization of Escherichia coli O157:H7 Strains Isolated from the One-Humped Camel (Camelus dromedarius) by Using Microarray DNA Technology

• Published in: Molecular biotechnology Vol. 51; no. 3; pp. 283 - 288
• Main Authors: Salehi, Taghi Zahraei, Tonelli, Alfreda, Mazza, Alberto, Staji, Hamid, Badagliacca, Pietro, Tamai, Iradj Ashrafi, Jamshidi, Reza, Harel, Josée, Lelli, Rossella, Masson, Luke
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.07.2012; Humana Press Inc; Springer; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biological and medical sciences; Biological Techniques; Biotechnology; Camelidae; Camelus; Camelus dromedarius; Cell Biology; Chemistry; Chemistry and Materials Science; Deoxyribonucleic acid; DNA; DNA microarrays; DNA, Bacterial - genetics; dromedaries; E coli; Enterocytes; Escherichia coli; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Escherichia coli O157; Escherichia coli O157 - classification; Escherichia coli O157 - genetics; Escherichia coli O157 - isolation & purification; Experimental allergic encephalomyelitis; feces; Feces - microbiology; Fundamental and applied biological sciences. Psychology; genes; Genes, Bacterial; Genetics; Human Genetics; LEE gene; loci; microarray technology; Microbiology; Oligonucleotide Array Sequence Analysis - methods; oligonucleotide probes; Oligonucleotides; Pathology; pathotypes; Plasmids; Probes; Protein Science; Serotypes; Shiga toxin; Toxins; Virulence; Virulence Factors - genetics; EHEC; STEC; Camelidae; O157:H7; Microarray; Camelus dromedarius; Escherichia coli; Characterization; Vertebrata; Mammalia; Technology; DNA; Bacteria; Genetics; Artiodactyla; Ungulata; Escherichia coli O157:H7; Enterobacteriaceae
• ISSN: 1073-6085; 1559-0305
• DOI: 10.1007/s12033-011-9466-7

From the Camelidae family members, several serotypes of Escherichia coli (E. coli) have recently been isolated from diarrhoeic and non-diarrhoeic faecal samples. To date Shiga toxin-producing E. coli (STEC) strains have never been typed in one-humped camel (Camelus dromedarius). In the present study, two E. coli O157:H7 strains isolated from sick dromedaries were investigated. Virulence gene profiles were determined using a custom E. coli virulence DNA microarray, composed of 70-mer oligonucleotide probes targeting 264 virulence or related genes of known E. coli pathotypes. Both strains displayed positive hybridization signals for the Locus of enterocyte effacement (LEE) gene probes (ler, eae, espA, espB, tir genes), two Shiga toxin probes (stx1 and stx2), the O157 O-antigen specific probe, various virulence plasmid (pO157) probes like katP in addition to other accessory virulence genes characterized in STEC.