Age, Wound Size, and Position of Injury - Dependent Vascular Regeneration Assay in Growing Leaves

Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf vasculature remain unexplored. Recently, we establ...

Full description

Saved in:
Bibliographic Details
Published inBio-protocol Vol. 11; no. 9; p. e4010
Main Authors Radhakrishnan, Dhanya, Shanmukhan, Anju Pallipurath, Kareem, Abdul, Mathew, Mabel Maria, Varaparambathu, Vijina, Aiyaz, Mohammed, Radha, Raji Krishna, Mekala, Krishnaprashanth Ramesh, Shaji, Anil, Prasad, Kalika
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.05.2021
Bio-protocol LLC
Subjects
Biology
Methods
Arabidopsis regeneration
Leaf incision
Vascular regeneration
Wound size
Age-dependent
Online AccessGet full text

Cover

More Information
Summary:Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf vasculature remain unexplored. Recently, we established a new vascular regeneration assay in growing leaves and discovered the underlying molecular mechanism. Here, we describe the detailed stepwise method for the incision and regeneration assay used to study leaf vascular regeneration. By using a combination of micro-surgical perturbations, brightfield microscopy, and other experimental approaches, we further show that the age of the leaf as well as the position and size of the injury determine the overall success rate of regeneration. This easy-to-master vascular regeneration assay is an efficient and rapid method to study the mechanism of vascular regeneration in growing leaves. The assay can be readily combined with cellular and molecular biology techniques.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Present Address: School of Life and Environmental Sciences, The University of Sydney, NSW, Australia
Contributed equally to this work
Present address: Microbiology and Fermentation Technology Department, CSIR - Central Food Technological Research Institute, Mysuru, Karnataka 570020, India
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.4010