Age, Wound Size, and Position of Injury - Dependent Vascular Regeneration Assay in Growing Leaves
Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf vasculature remain unexplored. Recently, we establ...
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Published in | Bio-protocol Vol. 11; no. 9; p. e4010 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Bio-Protocol
05.05.2021
Bio-protocol LLC |
Subjects | |
Online Access | Get full text |
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Summary: | Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf vasculature remain unexplored. Recently, we established a new vascular regeneration assay in growing leaves and discovered the underlying molecular mechanism. Here, we describe the detailed stepwise method for the incision and regeneration assay used to study leaf vascular regeneration. By using a combination of micro-surgical perturbations, brightfield microscopy, and other experimental approaches, we further show that the age of the leaf as well as the position and size of the injury determine the overall success rate of regeneration. This easy-to-master vascular regeneration assay is an efficient and rapid method to study the mechanism of vascular regeneration in growing leaves. The assay can be readily combined with cellular and molecular biology techniques. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present Address: School of Life and Environmental Sciences, The University of Sydney, NSW, Australia Contributed equally to this work Present address: Microbiology and Fermentation Technology Department, CSIR - Central Food Technological Research Institute, Mysuru, Karnataka 570020, India |
ISSN: | 2331-8325 2331-8325 |
DOI: | 10.21769/BioProtoc.4010 |