Characterization of a putative transcription factor gene expressed in the 20-OH-ecdysone inducible puff 74EF in Drosophila melanogaster

• Published in: Nucleic acids research Vol. 17; no. 12; pp. 4455 - 4464
• Main Authors: Janknecht, R, Taube, W, Ludecke, H.J, Pongs, O
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 26.06.1989
• Subjects: 74e protein; alternative splicing; Amino Acid Sequence; amino acid sequences; Animals; Base Sequence; Biological and medical sciences; Blotting, Northern; Cloning, Molecular; complementary DNA; Diptera; DNA - isolation & purification; DNA-binding proteins; Drosophila melanogaster; Drosophila melanogaster - genetics; Drosophila melanogaster - growth & development; Drosophila Proteins; Drosophilidae; Ecdysone - physiology; ecdysterone; Fundamental and applied biological sciences. Psychology; genbank/x15087; gene expression; Gene Expression Regulation; Genes. Genome; genetic regulation; Insect Hormones - genetics; Insect Hormones - isolation & purification; messenger RNA; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; nucleotide sequences; Peptide Elongation Factors - genetics; structural genes; transcription (genetics); Transcription Factors - genetics; Transcription, Genetic; Nucleotide sequence; Insecta; Homology; Gene expression; Ecdysone β; Drosophilidae; Gene; Arthropoda; DNA; Invertebrata; Molecular cloning; Transcription factor; Drosophila melanogaster; Diptera; Aminoacid sequence
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/17.12.4455

Drosophila melanogaster DNA has been cloned which encompasses the major part of the 20-OH-ecdysone inducible puff 74EF. One 20-OH-ecdysone responsive transcription unit was detected which gives rise to two alternative transcripts. The expression of one transcript in salivary glands of 3rd instar larvae is correlated with the 20-OH-ecdysone induced activity of puff 74EF. Corresponding cDNA analysis indicates that the two transcripts are translated into two different proteins which have alternative amino terminal ends. The carboxy terminal domain of the 74E proteins is similar to the carboxy terminal sequences of ets-oncoproteins suggesting that the 74E proteins represent alternative transcription factors. It is proposed that the activity of the 20-OH-ecdysone inducible puff 74EF leads to a switch in the synthesis of a transcription factor.