Production of Nitric Oxide in host-virus interaction: A case study with a compatible Begomovirus-Kenaf host-pathosystem

• Published in: Plant signaling & behavior Vol. 5; no. 6; pp. 668 - 676
• Main Authors: Sarkar, Tuhin Subhra, Majumdar, Uddalak, Roy, Anirban, Maiti, Debasis, Goswamy, Achintya Mohan, Bhattacharjee, Arindam, Ghosh, Subrata Kumar, Ghosh, Sanjay
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.06.2010; Landes Bioscience
• Subjects: alanine; ammonia; Binding; Biology; Bioscience; Calcium; Cancer; case studies; Cell; Cycle; disease resistance; Hibiscus - enzymology; Hibiscus - metabolism; Hibiscus - virology; Landes; leaves; Mosaic Viruses - physiology; nitric oxide; Nitric Oxide - metabolism; nitrogen; Nitrosation; Organogenesis; pathogenesis; Phenylalanine Ammonia-Lyase - metabolism; plant diseases and disorders; Plant Viruses - physiology; Proteins; Reactive Nitrogen Species - metabolism; Research Paper; shoots; tyrosine
• ISSN: 1559-2324; 1559-2316; 1559-2324
• DOI: 10.4161/psb.5.6.11282

Nitric oxide (NO) plays a key role in plant diseases resistance. Here we have first time demonstrated that begomovirus infection in susceptible H. cannabinus plants, results in elevated NO and reactive nitrogen species production during early infection stage not only in infected leaf but also in root and shoot. Production of NO was further confirmed by oxyhemoglobin assay. Furthermore, we used Phenyl alanine ammonia lyase as marker of pathogenesis related enzyme. In addition evidence for protein tyrosine nitration during the early stage of viral infection clearly showed the involvement of nitrosative stress.