Metabolic Enzyme Alterations and Astrocyte Dysfunction in a Murine Model of Alexander Disease With Severe Reactive Gliosis

• Published in: Molecular & cellular proteomics Vol. 21; no. 1; p. 100180
• Main Authors: Heaven, Michael R., Herren, Anthony W., Flint, Daniel L., Pacheco, Natasha L., Li, Jiangtao, Tang, Alice, Khan, Fatima, Goldman, James E., Phinney, Brett S., Olsen, Michelle L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2022; American Society for Biochemistry and Molecular Biology
• Subjects: Alexander disease; Alexander Disease - genetics; Alexander Disease - metabolism; Alexander Disease - pathology; Animals; astrocytes; Astrocytes - metabolism; Disease Models, Animal; Fabp7; Gliosis - metabolism; Gliosis - pathology; Humans; Mice; Mice, Transgenic; Mutation; Proteomics; reactive gliosis; Ugt8; PRM-MS; Mlc1; Plpp3; TFA; Gja1; Sqstm1; PPAR; Alexander disease; Bsn; Slc4a4; BCA; Acox1; Slc6a17; Prdx1; Slc6a11; Acox3; Pgd; Mag; Apoa1; Sorbs1; astrocytes; LPA; Cnp; CNS; MS2; PPP; Gclc; Gstm1; Fasn; Slc1a2; Mbp; Slc38a3; Gclm; Prdx6; Stxbp5l; Ugt8; Pck2; Ccnd2; reactive gliosis; Idh2; Cryl1; Sfxn5; GFAP; TIC; Snap25; Ca2; Dlg4; Ephx1; Ndrg2; Ctsd; Slc27a1; Hsp27; DAVID; Ilk; Rps27a; MDD; Cryab; Ddx3x; Txnrd1; μDIA; Gsto1; KEGG; TBI; LC3; Nadk2; Gpx1; Gpx3; Slc16a1; Acot1; Acot2; Rack1; Hmgcs2; Nfe2l2; Mobp; Ttyh1; Gan; Aldh1l1; Cat; Slc25a18; GABA; Acsl6; Acsl3; GAPDH; RFs; C1qb; C1qa; Gsta3; AxD; SRM; S1pr1; Ugp2; Cd44; Pygb; Mog; Acadl; Atp1b2; Gpr37l1; Fabp7; Gss; Gsr; Sparcl1; Kcnj10; Ntrk2; Stat3; Plp1; Vim; C1qc
• ISSN: 1535-9476; 1535-9484
• DOI: 10.1016/j.mcpro.2021.100180

Alexander disease (AxD) is a rare and fatal neurodegenerative disorder caused by mutations in the gene encoding glial fibrillary acidic protein (GFAP). In this report, a mouse model of AxD (GFAPTg;Gfap+/R236H) was analyzed that contains a heterozygous R236H point mutation in murine Gfap as well as a transgene with a GFAP promoter to overexpress human GFAP. Using label-free quantitative proteomic comparisons of brain tissue from GFAPTg;Gfap+/R236H versus wild-type mice confirmed upregulation of the glutathione metabolism pathway and indicated proteins were elevated in the peroxisome proliferator-activated receptor (PPAR) signaling pathway, which had not been reported previously in AxD. Relative protein-level differences were confirmed by a targeted proteomics assay, including proteins related to astrocytes and oligodendrocytes. Of particular interest was the decreased level of the oligodendrocyte protein, 2-hydroxyacylsphingosine 1-beta-galactosyltransferase (Ugt8), since Ugt8-deficient mice exhibit a phenotype similar to GFAPTg;Gfap+/R236H mice (e.g., tremors, ataxia, hind-limb paralysis). In addition, decreased levels of myelin-associated proteins were found in the GFAPTg;Gfap+/R236H mice, consistent with the role of Ugt8 in myelin synthesis. Fabp7 upregulation in GFAPTg;Gfap+/R236H mice was also selected for further investigation due to its uncharacterized association to AxD, critical function in astrocyte proliferation, and functional ability to inhibit the anti-inflammatory PPAR signaling pathway in models of amyotrophic lateral sclerosis (ALS). Within Gfap+ astrocytes, Fabp7 was markedly increased in the hippocampus, a brain region subjected to extensive pathology and chronic reactive gliosis in GFAPTg;Gfap+/R236H mice. Last, to determine whether the findings in GFAPTg;Gfap+/R236H mice are present in the human condition, AxD patient and control samples were analyzed by Western blot, which indicated that Type I AxD patients have a significant fourfold upregulation of FABP7. However, immunohistochemistry analysis showed that UGT8 accumulates in AxD patient subpial brain regions where abundant amounts of Rosenthal fibers are located, which was not observed in the GFAPTg;Gfap+/R236H mice. [Display omitted] •Models of AxD provide insight into functions of astrocytes related to neuropathology.•PPAR pathway found upregulated in AxD mice may be neuroprotective. Fatty acid binding protein (Fabp7) was upregulated in AxD mice and human CNS samples.•Depleted levels in the classical constituents of myelin were observed in AxD mice.•UGT8 may be related to myelin deficits in AxD. The article contains the first whole brain proteomic survey from a mouse model of Alexander disease (AxD). Several novel findings include activation of the PPAR signaling pathway, which has been reported to be protective in models of amyotrophic lateral sclerosis (ALS). Another finding related to the gliosis phenotype in AxD mice was the upregulation of fatty acid binding protein 7 (FABP7), which induces an NF-κB inflammatory response and counteracts the anti-inflammatory effects of PPAR signaling.