Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus

We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus, which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent‐protein based transcriptional reporter constructs for P. p...

Full description

Saved in:
Bibliographic Details
Published inGenesis (New York, N.Y. : 2000) Vol. 47; no. 5; pp. 300 - 304
Main Authors Schlager, Benjamin, Wang, Xiaoyue, Braach, Georg, Sommer, Ralf J.
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2009
Subjects
Amino Acid Sequence
Animals
Cloning, Molecular
collagen
Collagen - genetics
DNA, Helminth - genetics
DNA-mediated transformation
Genes, Dominant
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Helminth Proteins - genetics
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Molecular Sequence Data
Mutation
Nematoda
Nematoda - genetics
nematodes
parasite
Plasmids - genetics
Pristionchus pacificus
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Red Fluorescent Protein
Transformation, Genetic
Transgenes - genetics
Online AccessGet full text

Cover

More Information
Summary:We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus, which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent‐protein based transcriptional reporter constructs for P. pacificus. genesis 47:300–304, 2009. © 2009 Wiley‐Liss, Inc.
Bibliography:ark:/67375/WNG-TBBJ1XGB-8
istex:1EC45F68E7B106E39306B8161496D3FB1836CC3A
ArticleID:DVG20499
Benjamin Schlager and Xiaoyue Wang contributed equally to this work.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1526-954X
1526-968X
DOI:10.1002/dvg.20499