Quantitative, phenotypic, and functional evaluation of basophils in myelodysplastic syndromes
Background The myelodysplastic syndromes (MDS) are a group of clonal haematological disorders characterized by cytopenia(s), reduced differentiation‐capacity of myeloid cells, and impaired leukocyte function. However, little is known so far about basophil granulocytes in MDS. Design We have compared...
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Published in | European journal of clinical investigation Vol. 31; no. 10; pp. 894 - 901 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Science Ltd
01.10.2001
Blackwell Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Background The myelodysplastic syndromes (MDS) are a group of clonal haematological disorders characterized by cytopenia(s), reduced differentiation‐capacity of myeloid cells, and impaired leukocyte function. However, little is known so far about basophil granulocytes in MDS.
Design We have compared the numbers, phenotype and function of basophils in MDS patients with those in healthy subjects. A total numer of 23 patients with MDS (refractory anaemia, n = 8; refractory anaemia with ringsideroblasts, n = 7; refractory anaemia with excess of blasts/refractory anaemia with excess of blasts in transformation, n = 8) and 20 healthy donors were included.
Results The numbers of blood basophils in MDS patients (34·6 ± 62·9 μL−1) was lower compared to healthy controls (58·6 ± 64·9 μL−1). Correspondingly, whole blood histamine levels were lower in MDS patients (MDS 34·1 ± 29·1 ng mL−1 vs. normal donors 72·0 ± 36·9 ng mL−1). Like ‘normal’ basophils, basophils in MDS expressed interleukin‐3 receptor α (CD123), E‐NPP3 (CD203c), CR1 (CD35), CR3 (CD11b), CR4 (CD11c), membrane co‐factor protein (CD46), decay‐accelerating factor (CD55) and membrane attack complex inhibitory factor (CD59), as well as receptors for C3a, C5a (CD88), and IgE. Recombinant human (rh) C5a and anti‐IgE induced significant release of histamine from basophils in both groups of donors without significant differences between MDS and healthy controls.
Conclusions The absolute numbers of basophils in MDS patients are lower than in normal donors. However, basophils in MDS do not differ from their ‘normal counterparts’ in terms of complement receptor expression, IgE‐receptor expression, or functional responses to respective ligands. |
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Bibliography: | ArticleID:ECI887 ark:/67375/WNG-M5M2KWPF-2 istex:682A3C0BCB63E205B618DF81454A327AE51E2A41 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0014-2972 1365-2362 |
DOI: | 10.1046/j.1365-2362.2001.00887.x |