MR Susceptibility Separation for Quantifying Lesion Paramagnetic and Diamagnetic Evolution in Relapsing–Remitting Multiple Sclerosis

• Published in: Journal of magnetic resonance imaging Vol. 60; no. 5; pp. 1867 - 1879
• Main Authors: Zhu, Ziyan, Naji, Nashwan, Esfahani, Javad Hamidi, Snyder, Jeff, Seres, Peter, Emery, Derek J., Noga, Michelle, Blevins, Gregg, Smyth, Penelope, Wilman, Alan H.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.11.2024; Wiley Subscription Services, Inc
• Subjects: Adult; Brain - diagnostic imaging; Brain - pathology; Correlation coefficient; Correlation coefficients; Diamagnetism; Evolution; Female; Field strength; Humans; Image Processing, Computer-Assisted - methods; lesion evolution; Lesions; Longitudinal Studies; Magnetic resonance imaging; Magnetic Resonance Imaging - methods; magnetic susceptibility separation; Male; Mapping; Middle Aged; Multiple sclerosis; Multiple Sclerosis, Relapsing-Remitting - diagnostic imaging; Myelin; Prospective Studies; quantitative susceptibility mapping; Recovery; Reproducibility of Results; Separation; Statistical analysis; Statistical tests; Substantia alba; Susceptibility; Variation; White Matter - diagnostic imaging; White Matter - pathology; quantitative susceptibility mapping; magnetic susceptibility separation; lesion evolution; multiple sclerosis
• ISSN: 1053-1807; 1522-2586; 1522-2586
• DOI: 10.1002/jmri.29266

Background Multiple sclerosis (MS) lesion evolution may involve changes in diamagnetic myelin and paramagnetic iron. Conventional quantitative susceptibility mapping (QSM) can provide net susceptibility distribution, but not the discrete paramagnetic and diamagnetic components. Purpose To apply susceptibility separation (χ separation) to follow lesion evolution in MS with comparison to R2*/R2′/QSM. Study Type Longitudinal, prospective. Subjects Twenty relapsing–remitting MS subjects (mean age: 42.5 ± 9.4 years, 13 females; mean years of symptoms: 4.3 ± 1.4 years). Field Strength/Sequence Three‐dimensional multiple echo gradient echo (QSM and R2* mapping), two‐dimensional dual echo fast spin echo (R2 mapping), T2‐weighted fluid attenuated inversion recovery, and T1‐weighted magnetization prepared gradient echo sequences at 3 T. Assessment Data were analyzed from two scans separated by a mean interval of 14.4 ± 2.0 months. White matter lesions on fluid‐attenuated inversion recovery were defined by an automatic pipeline, then manually refined (by ZZ/AHW, 3/25 years' experience in MRI), and verified by a radiologist (MN, 25 years' experience in MS). Susceptibility separation yielded the paramagnetic and diamagnetic susceptibility content of each voxel. Lesions were classified into four groups based on the variation of QSM/R2* or separated into positive/negative components from χ separation. Statistical Tests Two‐sample paired t tests for assessment of longitudinal differences. Spearman correlation coefficients to assess associations between χ separation and R2*/R2′/QSM. Significant level: P < 0.005. Results A total of 183 lesions were quantified. Categorizing lesions into groups based on χ separation demonstrated significant annual changes in QSM//R2*/R2′. When lesions were grouped based on changes in QSM and R2*, both changing in unison yielded a significant dominant paramagnetic variation and both opposing yielded a dominant diamagnetic variation. Significant Spearman correlation coefficients were found between susceptibility‐sensitive MRI indices and χ separation. Data Conclusion Susceptibility separation changes in MS lesions may distinguish and quantify paramagnetic and diamagnetic evolution, potentially providing additional insight compared to R2* and QSM alone. Level of Evidence 2 Technical Efficacy Stage 2