Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum

• Published in: FEMS microbiology letters Vol. 309; no. 2; pp. 130 - 135
• Main Authors: Kim, Byung-Chun, Lee, Kang Hyun, Kim, Mi Na, Lee, Jongtae, Shin, Kee-Sun
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.08.2010; Blackwell Publishing Ltd; Oxford University Press
• Subjects: Bacteroidetes - classification; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Bacteroidetes - metabolism; Casein; Catalase; Cellulose; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Doraji; Esculin; Fatty acids; Fatty Acids - metabolism; Galactosidase; Gene sequencing; Menaquinones; Microbiology; Molecular Sequence Data; Mucilaginibacter dorajii; New species; Phylogenetics; Phylogeny; Platycodon - microbiology; Platycodon grandiflorum; Rhizosphere; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sodium chloride; Soil Microbiology; Species classification; Starch; Tyrosine; β-Galactosidase; Doraji; rhizosphere
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2010.02026.x

A Gram-negative, nonmotile and rod-shaped bacterial strain was isolated from the rhizosphere of Platycodon grandiflorum in a study of bacterial diversity, and its taxonomic position was investigated by a genotypic and phenotypic analysis. This isolate, designated as DR-f4, grew at 4-30 °C (optimally at 20-25 °C) and in the presence of 0-1% (w/v) NaCl. It contained MK-7 as the predominant menaquinone. The isolate had activities of catalase, oxidase and β-galactosidase and hydrolyzed aesculin, casein, carboxymethyl-cellulose, starch and l-tyrosine. The major cellular fatty acids were summed feature 3 (C₁₆:₁ω7c and/or iso-C₁₅:₀ 2OH) and iso-C₁₅:₀. The DNA G+C content was 42.6 mol%. This isolate belonged to the genus Mucilaginibacter based on phylogenetic analysis using 16S rRNA gene sequences. The nearest phylogenetic neighbors of strain DR-f4T were Mucilaginibacter lappiensis ANJL12T and Mucilaginibacter rigui WPCB133T, with 16S rRNA gene sequence similarity levels of 96.9% and 96.4%, respectively. The genotypic and phenotypic evidence suggests that strain DR-f4T should be classified as a novel species, for which the name Mucilaginibacter dorajii sp. nov. is proposed. The type strain for the novel species is DR-f4T (=KACC 14556T=JCM 16601T).