Separate optogenetic manipulation of Nerve/glial antigen 2 (NG2) glia and mural cells using the NG2 promoter

• Published in: Glia Vol. 71; no. 2; pp. 317 - 333
• Main Authors: Oishi, Mitsuhiro, Passlick, Stefan, Yamazaki, Yoshihiko, Unekawa, Miyuki, Adachi, Ruka, Yamada, Mayumi, Imayoshi, Itaru, Abe, Yoshifumi, Steinhäuser, Christian, Tanaka, Kenji F.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.02.2023; Wiley Subscription Services, Inc
• Subjects: Animals; Antigens; Antigens - genetics; Antigens - metabolism; Blood flow; cAMP; Cell activation; Cerebral blood flow; Cerebrum; Controllability; Depolarization; DM20; Hippocampus; In vivo methods and tests; Mice; Mice, Transgenic; mural cell; Nerves; Neuroglia - metabolism; oligodendrocyte precursor cell; Optogenetics; Subtraction; Tetracyclines - metabolism; tet‐system; Transgenic mice; oligodendrocyte precursor cell; cAMP; tet-system; mural cell; DM20
• ISSN: 0894-1491; 1098-1136
• DOI: 10.1002/glia.24273

Nerve/glial antigen 2 (NG2) is a protein marker of NG2 glia and mural cells, and NG2 promoter activity is utilized to target these cells. However, the NG2 promoter cannot target NG2 glia and mural cells separately. This has been an obstacle for NG2 glia‐specific manipulation. Here, we developed transgenic mice in which either cell type can be targeted using the NG2 promoter. We selected a tetracycline‐controllable gene induction system for cell type‐specific transgene expression, and generated NG2‐tetracycline transactivator (tTA) transgenic lines. We crossed tTA lines with the tetO‐ChR2 (channelrhodopsin‐2)‐EYFP line to characterize tTA‐dependent transgene induction. We isolated two unique NG2‐tTA mouse lines: one that induced ChR2‐EYFP only in mural cells, likely due to the chromosomal position effect of NG2‐tTA insertion, and the other that induced it in both cell types. We then applied a Cre‐mediated set‐subtraction strategy to the latter case and eliminated ChR2‐EYFP from mural cells, resulting in NG2 glia‐specific transgene induction. We further demonstrated that tTA‐dependent ChR2 expression could manipulate cell function. Optogenetic mural cell activation decreased cerebral blood flow, as previously reported, indicating that tTA‐mediated ChR2 expression was sufficient to impact cellular function. ChR2‐mediated depolarization was observed in NG2 glia in acute hippocampal slices. In addition, ChR2‐mediated depolarization of NG2 glia inhibited their proliferation but promoted their differentiation in juvenile mice. Since the tTA–tetO combination is expandable, the mural cell–specific NG2‐tTA line and the NG2 glia‐specific NG2‐tTA line will permit us to conduct observational and manipulation studies to examine in vivo function of these cells separately. Main Points Two NG2‐tTA transgenic mouse lines were generated: 1) mural cell‐ and 2) NG2 glia‐specific. Electrophysiological and cerebral blood flow experiments showed ChR2 functionality. NG2 glial depolarization inhibited proliferation but promoted differentiation.