Seropositivity to Helicobacter pylori heat shock protein 60 is strongly associated with intensity of chronic inflammation, particularly in antrum mucosa: an extension of an 18-year follow-up study of chronic gastritis in Saaremaa, Estonia

• Published in: FEMS immunology and medical microbiology Vol. 30; no. 2; pp. 143 - 149
• Main Authors: Vorobjova, Tamara, Ananieva, Olga, Maaroos, Heidi-Ingrid, Sipponen, Pentti, Villako, Kaljo, Utt, Meeme, Nilsson, Ingrid, Wadström, Torkel, Uibo, Raivo
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.2001; Blackwell
• Subjects: Antrum; Atrophy; Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Biological and medical sciences; Biomarkers - analysis; Chaperonin 60 - analysis; Chaperonin 60 - immunology; Chronic Disease; Chronic inflammation; Corpus mucosa; Estonia; Female; Follow-Up Studies; Gastritis - immunology; Gastritis - metabolism; Gastritis - physiopathology; Helicobacter pylori; Helicobacter pylori heat shock protein 60; Hsp60 protein; Human bacterial diseases; Humans; IgG antibody titer; Immunoglobulin G - analysis; Infectious diseases; Inflammation - etiology; Inflammation - metabolism; Long‐term follow‐up of chronic gastritis; Male; Medical sciences; Middle Aged; Mucous Membrane - metabolism; Prognosis; Pyloric Antrum - metabolism; Pyloric Antrum - pathology; Serologic Tests; Estonia; IgG antibody titer; heat shock protein 60; Long-term follow-up of chronic gastritis; Antrum; Chronic inflammation; Corpus mucosa; Human; Prognosis; Spirillales; Antibody; Spirillaceae; Inflammation; Serology; Enzyme immunoassay; Gastritis; Infection; Chronic; Helicobacter pylori; Follow up study; Heat shock protein; Bacteriosis; Digestive diseases; Bacteria; ELISA assay; Gastric disease
• ISSN: 0928-8244; 1574-695X
• DOI: 10.1111/j.1574-695X.2001.tb01563.x

Abstract Helicobacter pylori is a cause of chronic gastritis and leads to development of atrophy in some cases. There is evidence that the heat shock protein 60 (HSP60) of H. pylori is involved in induction of chronic inflammation. Seroprevalence of IgG antibodies to H. pylori HSP60 in an adult cohort from Saaremaa, Estonia (68 persons, median age 57 years), with a high prevalence of antibodies to cell surface proteins of H. pylori (92%) and a well characterized dynamics of chronic gastritis in an 18-year follow-up study, was tested using purified H. pylori HSP60 at a concentration of 1 µg ml−1 with ELISA. The state of the gastric mucosa and the presence of H. pylori in histological sections in the samples of 1979 and 1997 were assessed in accordance with the Sydney system. Seropositivity for H. pylori HSP60 was 65%. Immunological response to H. pylori HSP60 is associated with the morphological presence of H. pylori in the antrum and corpus (P=0.01) and is strongly correlated with the grade of chronic inflammation, particularly in the antrum mucosa (r=0.34; P=0.003; OR=5.97 (95% CI 1.21–29.3)), but is not associated with development of atrophy during 18 years of follow-up, or with the activity of gastritis. This finding supports the evidence that immunological response to H. pylori HSP60 may play a role in triggering of the inflammatory process in the gastric mucosa.