An Experimental Study on the Addition of Bacteria to Residual Anticancer Drugs: Evaluation of the Effect on Bacterial Growth

• Published in: Japanese Journal of Infectious Diseases Vol. 77; no. 2; pp. 61 - 67
• Main Authors: Kikuchi, Ken, Miyauchi, Rei, Yamaguchi, Tomoya, Sugiura, Hayato, Nogami, Taishi, Inoue, Yuki, Sato, Haruna, Sato, Hideki, Fujiwara, Nagatoshi, Maeda, Shinji
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases 29.03.2024; Japan Science and Technology Agency
• Subjects: 5-Fluorouracil; Anti-Bacterial Agents - pharmacology; Antibacterial activity; anticancer agents; Anticancer properties; Antiinfectives and antibacterials; Antineoplastic Agents - pharmacology; Antineoplastic drugs; Bacteria; bacterial contamination; Cancer; Carboplatin; Drug development; Drugs; E coli; Enterococcus faecalis; Escherichia coli; Etoposide; Fluorouracil - pharmacology; Irinotecan; Irinotecan - pharmacology; Methotrexate; Methotrexate - pharmacology; Microbial Sensitivity Tests; Microorganisms; Minimum inhibitory concentration; Paclitaxel; Protein biosynthesis; Protein synthesis; Proteins; residual medicine; Serratia marcescens; Staphylococcus aureus; antibacterial activity; bacterial contamination; anticancer agents; residual medicine
• ISSN: 1344-6304; 1884-2836
• DOI: 10.7883/yoken.JJID.2023.270

Using anticancer drugs as examples, we examined the possibility of reusing residual drugs. The use of residual drugs is not widespread owing to concerns regarding bacterial contamination. We combined anticancer drugs and bacteria to investigate their effects on bacterial growth. The anticancer drugs carboplatin, paclitaxel, etoposide, irinotecan, methotrexate, and 5-fluorouracil (5-FU) were mixed with Staphylococcus aureus, Enterococcus faecalis, Serratia marcescens, and Escherichia coli. After a certain period, the bacteria were counted. Irinotecan showed no antibacterial activity, whereas 5-FU exhibited high antibacterial activity against the tested bacteria. The 5-FU also showed a minimum inhibitory concentration value in the range of 8–80 μg/mL, depending on the bacterial species. 5-FU dose-dependently inhibited S. aureus growth at more than 0.8 µg/mL. Because protein synthesis systems are reportedly antibiotic targets, we used a cell-free protein synthesis system to confirm the mechanism of the antibacterial activity of the anticancer agent. 5-FU and methotrexate had direct inhibitory effects on protein synthesis. It has been suggested that even if residual drugs are contaminated with bacteria, there will be no microbial growth, or the microbes will be killed by the drug. With careful monitoring, 5-FU can potentially be used for antimicrobial purposes.