Hemoglobin-Conjugated Gold Nanoclusters for Qualitative Analysis of Haptoglobin Phenotypes

Designing a facile and rapid detection method for haptoglobin (Hp) phenotypes in human blood plasma is urgently needed to meet clinic requirements in hemolysis theranostics. In this work, a novel approach to qualitatively analyze Hp phenotypes was developed using a fluorescent probe of gold nanoclus...

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Published inPolymers Vol. 12; no. 10; p. 2242
Main Authors Tan, Shih-Hua, Yougbaré, Sibidou, Chu, Hsueh-Liang, Kuo, Tsung-Rong, Cheng, Tsai-Mu
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 29.09.2020
MDPI
Subjects
Apoptosis
Aqueous solutions
Biocompatibility
Blood plasma
Brain cancer
Charge transfer
Cytotoxicity
Fluorescent indicators
Fourier transforms
Glucose
Gold
gold nanoclusters
haptoglobin
Hemoglobin
Ligands
Microscopy
Nanoclusters
Nanoparticles
Oxidation
phenotypes
Qualitative analysis
Software
Spectrum analysis
St Louis Missouri
Canada
United States > US
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Summary:Designing a facile and rapid detection method for haptoglobin (Hp) phenotypes in human blood plasma is urgently needed to meet clinic requirements in hemolysis theranostics. In this work, a novel approach to qualitatively analyze Hp phenotypes was developed using a fluorescent probe of gold nanoclusters (AuNCs). Hemoglobin-conjugated (Hb)-AuNCs were successfully synthesized with blue-green fluorescence and high biocompatibility via one-pot synthesis. The fluorescence of Hb-AuNCs comes from the ligand-metal charge transfer between surface ligands of Hb and the gold cores with high oxidation states. The biocompatibility assays including cell viability and fluorescence imaging, demonstrated high biocompatibility of Hb-AuNCs. For the qualitative analysis, three Hp phenotypes in plasma, Hp 1-1, Hp 2-1, and Hp 2-2, were successfully discriminated according to changes in the fluorescence intensity and peak position of the maximum intensity of Hb-AuNCs. Our work provides a practical method with facile and rapid properties for the qualitative analysis of three Hp phenotypes in human blood plasma.
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ISSN:2073-4360
2073-4360
DOI:10.3390/polym12102242