Combination of two-dimensional electrophoresis and shotgun peptide sequencing in comparative proteomics

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 815; no. 1; pp. 3 - 9
• Main Authors: Kubota, Kazuishi, Kosaka, Toshiyuki, Ichikawa, Kimihisa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.02.2005
• Subjects: Animals; Chromatography, Liquid - methods; Comparative proteomics; Electrophoresis, Gel, Two-Dimensional - methods; Genome, Human; Humans; Mass Spectrometry - methods; Peptide Fragments - chemistry; Peptide Fragments - isolation & purification; Peptide Mapping; Proteomics - methods; Shotgun peptide sequencing; Two-dimensional electrophoresis; Comparative proteomics; Shotgun peptide sequencing; Two-dimensional electrophoresis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2004.10.030

Two-dimensional electrophoresis (2-DE) and shotgun peptide sequencing are the two major technologies to compare the expression profile of proteins, which is also referred to as comparative proteomics or quantitative proteomics. Although the methodologies, such as difference gel electrophoresis for 2-DE and isotope-coded affinity tags for shotgun peptide sequencing, have made rapid progress, these two approaches have their own strengths and weaknesses. Therefore, the combination of the two methodologies is beneficial for the purpose of better comparative proteomics, especially in comprehensive coverage of the proteome and protein information such as post-translational modifications.