Antimicrobial activities of ethanol and butanol fractions of white rose petal extract

• Published in: Regulatory toxicology and pharmacology Vol. 76; pp. 57 - 62
• Main Authors: Park, Dongsun, Shin, Kyungha, Choi, Youngjin, Guo, Haiyu, Cha, Yeseul, Kim, Sa-Hyun, Han, Nam Soo, Joo, Seong Soo, Choi, Jae Kwon, Lee, Yoon Bok, Choi, Ehn-Kyoung, Kim, Jong Bae, Kim, Yun-Bae
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.04.2016
• Subjects: Animals; Anti-Infective Agents - isolation & purification; Anti-Infective Agents - pharmacology; Antimicrobial activity; Butanol fraction; Butanols - chemistry; Colony Count, Microbial; Disease Models, Animal; Dose-Response Relationship, Drug; Ethanol - chemistry; Ethanol fraction; Flowers - chemistry; Gastric Mucosa - drug effects; Gastric Mucosa - microbiology; Helicobacter Infections - drug therapy; Helicobacter Infections - microbiology; Helicobacter pylori; Helicobacter pylori - drug effects; Helicobacter pylori - growth & development; Male; Mice, Inbred C57BL; Microbial Sensitivity Tests; Phytotherapy; Plants, Medicinal; Propionibacterium acnes; Propionibacterium acnes - drug effects; Propionibacterium acnes - growth & development; Rosa; Rosa - chemistry; Solvents - chemistry; White rose petal extract; White rose petal extract; Antimicrobial activity; Butanol fraction; Helicobacter pylori; Propionibacterium acnes; Ethanol fraction
• ISSN: 0273-2300; 1096-0295
• DOI: 10.1016/j.yrtph.2016.01.011

White rose (Rosa hybrida) petals were extracted with ethanol (EtOH) or butanol (BuOH), and tested for their antimicrobial activities against two species of Gram-positive bacteria, six species of Gram-negative bacteria, and two species of fungi. On in vitro antimicrobial assays, Helicobacter pylori and Propionibacterium acnes were highly susceptible to white rose petal extract (WRPE)-EtOH and WRPE-BuOH, leading to minimal inhibitory concentrations of 100 and 10 μg/mL for H. pylori and 400 and 40 μg/mL for P. acnes, respectively. In in vivo experiments, C57BL/6 mice were infected with H. pylori by intragastric inoculation (1 × 108 CFU/mouse) 3 times, and orally treated twice a day for 14 days with WRPE-EtOH and WRPE-BuOH. On a CLO kit assay, 200 mg/kg of WRPE-EtOH fully eliminated the bacteria from the gastric mucosa, and the effect of 100 mg/kg of ethanol fraction was similar to pantoprazole (30 mg/kg), displaying 75% elimination. WRPE-BuOH was more effective, exhibiting 75% elimination at 20 mg/kg. The CLO test results were confirmed by bacterial identification. WRPE-EtOH and WRPE-BuOH inhibited the growth of various bacteria and fungi, and in particular, they effectively killed H. pylori and eliminated the bacteria from the mouse stomach. The results indicate that WRPE-EtOH and WRPE-BuOH could be good candidates for the elimination of H. pylori. •In addition to the popular use of rose oils as perfumes, pharmacological activities of rose extracts have been reported.•White rose petal extracts (WRPE) exhibited antimicrobial activities.•Ethanol and butanol fractions of WRPE strongly inhibited the growth of Helicobacter pylori.•The fractions of WRPE effectively eliminated Helicobacter pylori from the mouse stomach.