Control of APN/CD13 and NEP/CD10 on sperm motility

• Published in: Asian journal of andrology Vol. 12; no. 6; pp. 899 - 902
• Main Authors: Subirán, Nerea, Pinto, Francisco M, Agirregoitia, Ekaitz, Candenas, Luz, Irazusta, Jon
• Format: Journal Article
• Language: English
• Published: China Medknow Publications & Media Pvt. Ltd 01.11.2010; Nature Publishing Group
• Subjects: Amino Acids - pharmacology; APN; CD13 Antigens - antagonists & inhibitors; CD13 Antigens - physiology; Humans; Imidazoles - pharmacology; Male; Neprilysin - antagonists & inhibitors; Neprilysin - physiology; Percoll; Short Communication; Sperm Motility - drug effects; Sperm Motility - physiology; Spermatozoa - enzymology; Thiorphan - pharmacology; 抑制剂; 精子活力; 精子细胞; 精子运动能力; 经济政策
• ISSN: 1008-682X; 1745-7262
• DOI: 10.1038/aja.2010.82

Aminopeptidase N （APN/CD13） and neutral endopeptidase （NEP/CD10） are enzymes present in human sperm cells and involved in regulation of sperm motility of noncapacitated spermatozoa. We investigated the involvement of APN/CD 13 and NEP/CD 10 in motility and in kinematic parameters of human capacitated spermatozoa. Sperm cells isolated by a discontinuous Percoll gradient （40%-80%） followed up by swim-up techniques were incubated with the APN/CD 13 -specific inhibitor, leuhistin （100 μmol L^-1）, and the NEP/CD 10-specific inhibitor, thiorphan （1 μmol L^-1）. The complete inhibition of both APN/CD 13 and NEP/CD 10 improved sperm motility. Spermatozoa incubated with the APN/CD13-specific inhibitor lenhistin showed asymmetrical trajectories, whereas sperm trajectories were more regular after treatment with the NEP/CD 10-specific inhibitor thiorphan. In conclusion, APN/CD 13 and NEP/CD 10 modulate the motility of capacitated spermatozoa, although each of the enzymes seems to participate in the control of different aspects of sperm motility. Therefore, both inhibitors may be useful for sperm activation at different functional stages of spermatozoa.