Molecular characterization of a troponin I-like protein from the hard tick Haemaphysalis longicornis

A cDNA expression library prepared from mRNA of Haemaphysalis longicornis ( H. longicornis) was screened with a H. longicornis-infested rabbit serum. A cDNA encoding 27/30 kDa proteins was cloned and designated P27/30 gene. The predicted amino acid sequence of the P27/30 gene shows a rather high hom...

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Published inInsect biochemistry and molecular biology Vol. 32; no. 1; pp. 67 - 73
Main Authors You, Myungjo, Xuan, Xuenan, Tsuji, Naotoshi, Kamio, Tsugihiko, Igarashi, Ikuo, Nagasawa, Hideyuki, Mikami, Takeshi, Fujisaki, Kozo
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.12.2001
Subjects
Amino Acid Sequence
Animals
Base Sequence
Disease Models, Animal
DNA, Complementary
Drosophila melanogaster
Escherichia coli
Gene Expression
Gene Library
Genetic Testing
Haemaphysalis longicornis
Ixodidae
Ixodidae - genetics
Ixodidae - metabolism
Mice
Microfilament Proteins - genetics
Microfilament Proteins - metabolism
Molecular Sequence Data
Molecular Weight
P27/30 gene
Rabbits
Sequence Analysis, DNA
Tick
Tick Infestations - parasitology
Troponin I
Troponin I - genetics
Troponin I - metabolism
Troponin I
Tick
Haemaphysalis longicornis
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Summary:A cDNA expression library prepared from mRNA of Haemaphysalis longicornis ( H. longicornis) was screened with a H. longicornis-infested rabbit serum. A cDNA encoding 27/30 kDa proteins was cloned and designated P27/30 gene. The predicted amino acid sequence of the P27/30 gene shows a rather high homology (58% amino acid identities and 11% amino acid similarity) with Drosophila melanogaster troponin I clone E2. H. longicornis P27/30 possesses amino acid sequence of actin-binding domains of troponin I at the amino acid residues 128–148, suggesting that H. longicornis P27/30 is a troponin I-like protein. By immunoblot analysis, mouse anti-recombinant P27/30 serum reacted with major constituent protein bands in extracts of adult ticks, and also immunoreacted with muscle, cuticle, gut, and salivary gland in H. longicornis ticks. Moreover, immunohistochemistry using the anti-P27/30 serum showed a strong reactivity in muscle, suggesting that native P27/30 is expressed abundantly in that tissue.
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ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(01)00081-9