Description of Sulfitobacter sediminilitoris sp. nov., isolated from a tidal flat

• Published in: FEMS microbiology letters Vol. 367; no. 9; pp. 1 - 6
• Main Authors: Park, Sooyeon, Chen, Siyu, Lee, Jung-Sook, Kim, Wonyong, Yoon, Jung-Hoon
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.05.2020
• Subjects: Deoxyribonucleic acid; Discovery and exploration; DNA; Environmental aspects; Fatty acids; Gene sequencing; Genetic aspects; Genomes; Genomic analysis; Gram-negative bacteria; Identification and classification; Lecithin; Lipids; Microbiology; New species; Nucleotide sequence; Phosphatidylcholine; Phosphatidylglycerol; Phylogenetics; Phylogeny; Proteobacteria; rRNA 16S; Strains (organisms); Streblonema porphyrae; Sulfitobacter; Tidal flats; Tidal marshes; Ubiquinone; South Korea; sp. nov; novel species; tidal flat; polyphasic taxonomy; Sulfitobacter sediminilitoris sp. nov
• ISSN: 1574-6968; 0378-1097; 1574-6968
• DOI: 10.1093/femsle/fnaa074

ABSTRACT A Gram-stain-negative bacterial strain, JBTF-M27T, was isolated from a tidal flat from Yellow Sea, Republic of Korea. Neighbor-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M27T fell within the clade comprising the type strains of Sulfitobacter species. Strain JBTF-M27T exhibited the highest 16S rRNA gene sequence similarity (98.8%) to the type strain of S. porphyrae. Genomic ANI and dDDH values of strain JBTF-M27T between the type strains of Sulfitobacter species were less than 76.1 and 19.2%, respectively. Mean DNA-DNA relatedness value between strain JBTF-M27T and the type strain of S. porphyrae was 21%. DNA G + C content of strain JBTF-M27T from genome sequence was 57.8% (genomic analysis). Strain JBTF-M27T contained Q-10 as the predominant ubiquinone and C18:1ω7c as the major fatty acid. The major polar lipids of strain JBTF-M27T were phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain JBTF-M27T is separated from recognized Sulfitobacter species. On the basis of the data presented, strain JBTF-M27T ( = KACC 21648T = NBRC 114356T) is considered to represent a novel species of the genus Sulfitobacter, for which the name Sulfitobacter sediminilitoris sp. nov. is proposed. Based on distinct polyphasic taxonomic evidences, novel strain JBTF-M27T represents a new species of the genus Sulfitobacter, for which the name Sulfitobacter sediminilitoris sp. nov. is proposed.