Impact of Freeze-Drying on Cord Blood (CB), Serum (S), and Platelet-Rich Plasma (CB-PRP) Preparations on Growth Factor Content and In Vitro Cell Wound Healing

• Published in: International journal of molecular sciences Vol. 23; no. 18; p. 10701
• Main Authors: Valente, Sabrina, Ciavarella, Carmen, Astolfi, Gloria, Bergantin, Elisa, Curti, Nico, Buzzi, Marina, Fontana, Luigi, Versura, Piera
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 14.09.2022; MDPI
• Subjects: Biological properties; Blood; Blood platelets; brain-derived growth factor; Brain-derived neurotrophic factor; Cell activation; Cell adhesion & migration; Cell cycle; Cell growth; Cord blood; Cornea; Enzyme-linked immunosorbent assay; epidermal growth factor; Eye disorders; Freeze drying; Gene expression; Glial cells; Glial fibrillary acidic protein; Growth factors; Morphology; Ophthalmology; Plasma; platelet-rich plasma; serum; Vimentin; Wound healing
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms231810701

Blood-based preparations are used in clinical practice for the treatment of several eye disorders. The aim of this study is to analyze the effect of freeze-drying blood-based preparations on the levels of growth factors and wound healing behaviors in an in vitro model. Platelet-rich plasma (PRP) and serum (S) preparations from the same Cord Blood (CB) sample, prepared in both fresh frozen (FF) and freeze-dried (FD) forms (and then reconstituted), were analyzed for EGF and BDNF content (ELISA Quantikine kit). The human MIO-M1 glial cell line (Moorfield/Institute of Ophthalmology, London, UK) was incubated with FF and FD products and evaluated for cell migration with scratch-induced wounding (IncuCyte S3 Essen BioScience), proliferation with cyclin A2 and D1 gene expression, and activation with vimentin and GFAP gene expression. The FF and FD forms showed similar concentrations of EGF and BDNF in both the S and PRP preparations. The wound healing assay showed no significant difference between the FF and FD forms for both S and PRP. Additionally, cell migration, proliferation, and activation did not appear to change in the FD forms compared to the FF ones. Our study showed that reconstituted FD products maintained the growth factor concentrations and biological properties of FF products and could be used as a functional treatment option.