Phylogenetic Relationships of Rhizobia Based on Citrate Synthase Gene Sequences

• Published in: Systematic and applied microbiology Vol. 27; no. 6; pp. 703 - 706
• Main Authors: Hernández-Lucas, Ismael, Rogel-Hernández, Marco Antonio, Segovia, Lorenzo, Rojas-Jiménez, Keilor, Martínez-Romero, Esperanza
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01.11.2004; Elsevier; Elsevier Science Ltd
• Subjects: 16S rRNA; Alphaproteobacteria - classification; Alphaproteobacteria - enzymology; Alphaproteobacteria - genetics; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Citrate (si)-Synthase - genetics; DNA, Bacterial - chemistry; DNA, Bacterial - isolation & purification; DNA, Ribosomal - chemistry; DNA, Ribosomal - isolation & purification; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genes, rRNA; gltA; Microbiology; Molecular Sequence Data; Phylogeny; Rhizobiaceae - classification; Rhizobiaceae - enzymology; Rhizobiaceae - genetics; Rhizobium; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; 16S rRNA; gltA; Phylogeny; Nucleotide sequence; Phylogenetic tree; Citrate; 16S-RNA; Bacteria; Rhizobiaceae
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1078/0723202042369893

Partial nucleotide sequences of the citrate synthase ( gltA) gene from different rhizobia genera were determined. Tree topologies based on this housekeeping gene were similar to that obtained using 16S rRNA sequences. However gltA appeared to be more reliable at determining phylogenetic relationships of closely related taxa. We propose gltA sequences as an additional tool to be used in molecular phylogenetic studies.