Bifunctional magnetic nanoparticles for analysis of aldehyde metabolites in exhaled breath of lung cancer patients

• Published in: Journal of Chromatography A Vol. 1324; pp. 29 - 35
• Main Authors: Xu, Hui, Wei, Yanling, Zhu, Linli, Huang, Jing, Li, Yu, Liu, Feilong, Wang, Shuling, Liu, Shi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10.01.2014; Elsevier
• Subjects: Aldehydes; Aldehydes - analysis; Aldehydes - metabolism; Biological; Biological and medical sciences; Breath Tests; Cancer; Condensates; Exhaled breath condensate; Extraction; Host-tumor relations. Immunology. Biological markers; Humans; Lung cancer; Lung Neoplasms - chemistry; Lung Neoplasms - metabolism; Magnetic solid-phase extraction; Magnetite Nanoparticles - chemistry; Magnetite Nanoparticles - ultrastructure; Medical sciences; Metabolites; Microscopy, Electron, Scanning; Nanoparticles; Patients; Polysorbates - chemistry; Reproducibility of Results; Solid Phase Extraction - methods; Surface-Active Agents - chemistry; Tumors; Magnetic solid-phase extraction; Aldehydes; Exhaled breath condensate; Lung cancer; Solid phase extraction; Chemical analysis; Metabolite; Nanoparticle; HPLC chromatography; Surfactant polymer; Alkanal; Derivatization; Chemical enrichment; Characterization; Sample preparation; Bronchus disease; Adsorbent; Quantitative analysis; Trace analysis; Human; Lung disease; Expired air; Iron II Iron III Oxides; Healthy subject; Respiratory disease; Non ionic surfactant; Patient; Malignant tumor; Aldehyde; Coated material; Magnetic particles; Magnetic separation; Preparation; Polysorbate; Comparative study; Cancer
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2013.11.041

•A bifunctional magnetic nanoparticles were synthesized.•The material is suitable for biological sample analysis.•A simple and sensitive method was developed for analysis of aldehydes in exhaled breath condensate. We report here the preparation of dual-functionalized magnetic nanoparticles, with the nanoparticles as extraction sorbents, a magnetic solid phase extraction method was developed and applied for the analysis of trace amount of aldehydes in human exhaled breath condensate. In the material, octyl-functionalized internal surface provided hydrophobic groups for extraction, non-ionic surfactant (Tween-20)-coated outer surface offered hydrophilic network structure to prevent the access of macromolecules, strong magnetic property of nanoparticles simplified the analytical procedure. The experimental results showed that the prepared nanoparticles exhibited good dispersibility in aqueous solution and excellent extraction efficiency toward aldehydes. Six aldehydes were derivatized with 2,4-dinitrophenylhydrazine and then the formed hydrazones were extracted by the nanoparticles and analyzed by high-performance liquid chromatography–photo diode array detector. Under the optimal conditions, the method provided low limits of detection (2.9–21.5nmolL−1), satisfactory reproducibility (relative standard deviations, 2.9–13.1%) and acceptable recoveries (73.7–133.1%). The developed method was applied successfully to determine the aldehydes metabolites in the exhaled breath condensate samples of healthy people and lung cancer patients. The dual-functionalized material is suitable for biological sample analysis. The proposed method provides an alternative approach for quantification of aldehyde metabolites in complex biological samples.