Albumin Might Attenuate Bacteria-Induced Damage on Kupffer Cells for Patients with Chronic Liver Disease

• Published in: Cells (Basel, Switzerland) Vol. 10; no. 9; p. 2298
• Main Authors: Lin, Hao, Fan, Yuhui, Wieser, Andreas, Zhang, Jiang, Regel, Ivonne, Nieß, Hanno, Mayerle, Julia, Gerbes, Alexander L., Steib, Christian J.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 03.09.2021; MDPI
• Subjects: Albumin; Bacteria; Bacterial infections; Biobanks; C-reactive protein; Cholangitis; chronic liver diseases; E coli; Endothelial cells; Gene expression; Gram-positive bacteria; hepatic non-parenchymal cells; Hepatitis; Human serum albumin; Immunofluorescence; Immunomodulation; Inflammation; Kinases; Kupffer cells; L-Lactate dehydrogenase; Lactic acid; Liver cirrhosis; Liver diseases; NF-κB protein; Patients; Proteins; Staphylococcus infections; Stellate cells; Streptococcus infections; United States > US; Germany
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells10092298

Chronic liver diseases (CLDs) are complex diseases that cause long-term inflammation and infection, which in turn accelerate their development. The usage of albumin in patients with CLDs has been debated for years. Human serum albumin (HSA) plays a key role in immunomodulation during the process of CLDs. The correlation between albumin and C-reactive protein (CRP) in CLD patients was analyzed by linear regression with the Pearson statistic. The damage of THP-1 and primary cells was evaluated by measuring the lactate dehydrogenase (LDH) in the supernatant. Immunofluorescence staining was performed to determine underlying pathways in Kupffer cells (KCs). Albumin negatively correlated with infection in patients with CLDs. In vitro experiments with THP-1 cells and KCs showed that albumin reduced LDH release after stimulation with bacterial products, while no differences in hepatic stellate cells (HSCs) and sinusoidal endothelial cells (SECs) were detected. Moreover, immunofluorescence staining revealed an increase of p-ERK and p-NF-kB p65 density after albumin treatment of KCs stimulated by bacterial products. In conclusion, albumin could assist CLD patients in alleviating inflammation caused by bacterial products and might be beneficial to patients with CLDs by securing KCs from bacteria-induced damage, providing a compelling rationale for albumin therapy in patients with CLDs.