AtSig5 is an essential nucleus-encoded Arabidopsis sigma-like factor

• Published in: Plant physiology (Bethesda) Vol. 132; no. 2; pp. 739 - 747
• Main Authors: Yao, J, Roy-Chowdhury, S, Allison, L.A
• Format: Journal Article
• Language: English
• Published: United States American Society of Plant Biologists 01.06.2003; The American Society for Plant Biologists
• Subjects: Alleles; Arabidopsis; Arabidopsis - genetics; Arabidopsis Proteins; Arabidopsis Proteins - genetics; Arabidopsis thaliana; AtSig 5 protein; Base Sequence; bioaccumulation; cell nucleus; Cell Nucleus - genetics; Chloroplasts; Development and Hormone Action; DNA; DNA Primers; DNA, Bacterial; DNA, Bacterial - genetics; DNA, Plant; DNA, Plant - genetics; flowers; Genes; genetics; insertional mutagenesis; leaves; messenger RNA; molecular sequence data; Mutagenesis, Insertional; nucleotide sequences; Ovules; phenotype; Phenotypes; plant proteins; Plants; Plastids; Polymerase chain reaction; Promoter Regions, Genetic; RNA; Sigma Factor; Sigma Factor - genetics; sigma factors; Transcription, Genetic; transfer DNA
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.102.017913

Transcription of chloroplast genes is subject to control by nucleus-encoded proteins. The chloroplast-encoded RNA polymerase (PEP) is a eubacterial-type RNA polymerase that is presumed to assemble with nucleus-encoded σ-factors mediating promoter recognition. Recently, families of σ-factor genes have been identified in several plants including Arabidopsis. One of these genes, Arabidopsis SIG5, encodes a σ-factor, AtSig5, which is phylogenetically distinct from the other family members. To investigate the role of this plant σ-factor, two different insertional alleles of the SIG5 gene were identified and characterized. Heterozygous mutant plants showed no visible leaf phenotype, but exhibited siliques containing aborted embryos and unfertilized ovules. Our inability to recover plants homozygous for a SIG5 gene disruption indicates that SIG5 is an essential gene. SIG5 transcripts accumulate in flower tissues, consistent with a role for AtSig5 protein in reproduction. Therefore, SIG5 encodes an essential member of the Arabidopsis σ-factor family that plays a role in plant reproduction in addition to its previously proposed role in leaf chloroplast gene expression.