A novel heptasialosyl c-series ganglioside in embryonic chicken brain: its structure and stage-specific expression

• Published in: Biochimica et biophysica acta Vol. 1571; no. 1; pp. 18 - 26
• Main Authors: Saito, Megumi, Kitamura, Hisayo, Sugiyama, Kiyoshi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.05.2002
• Subjects: A2B5; analysis; animal physiology; Animals; biosynthesis; Brain; Brain - embryology; Brain - metabolism; C-series ganglioside; Carbohydrate Sequence; chemistry; Chicken; Chickens; Chromatography, Thin Layer; Development; embryology; Ganglioside; Gangliosides; Gangliosides - analysis; Gangliosides - biosynthesis; Gangliosides - chemistry; Gangliosides - metabolism; Glycolipid; Glycosphingolipids; Glycosphingolipids - metabolism; Mass Spectrometry; metabolism; Molecular Sequence Data; Neuraminidase; Brain; MS; TLC; Glycolipid; C-series ganglioside; ECL; Ganglioside; Development; DMB; A2B5; Chicken; ESI; HPLC; CID
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/S0304-4165(02)00201-5

A ganglioside of unknown structure (ganglioside X) was purified from chicken brain at embryonic day 12 (E12) and characterized for its structure. Ganglioside X was reactive with a monoclonal antibody A2B5 and migrated below GH1c on thin-layer chromatography (TLC). Extensive treatment of ganglioside X with Clostridium perfringens sialidase produced a single ganglioside product. This ganglioside was identified as GM1 based upon its chromatographic mobility and reactivity to cholera toxin B subunit and anti-GM1 antibody. Partial hydrolysis of ganglioside X by sialidase generated several degradation products including GH1c, GP1c, and GQ1c. Electrospray ionization (ESI)-mass spectrometry (MS) of the permethylated derivative of ganglioside X produced a triple-charged parent ion peak at m/ z 1355, which corresponded with the gangliotetraose oligosaccharide structure having seven sialic acids and ceramide with the molecular mass of 566 (as non-methylated form). Collision-induced dissociation (CID)-MS 2 showed fragment ions including those at m/ z 1066 and 1931; these two ions matched the structures of (NeuAc) 3-Gal-Glc-Cer and (NeuAc) 4-Gal-GalNAc, respectively. These structures were confirmed by CID-MS 3 of the corresponding peaks. Based upon these findings, the structure of ganglioside X was identified as NeuAc-NeuAc-NeuAc-NeuAc-Galβ1–3GalNAcβ1–4(NeuAc-NeuAc-NeuAcα2–3)Galβ1–4Glcβ1-1′Cer. This ganglioside was designated as GS1c. A developmental study demonstrated that GS1c was expressed in chicken brain during a period from E6 to E13 and thereafter decreased rapidly in its concentration. The present study suggests that GS1c may play a specific role in early development of chicken brain.