Mucosal leishmaniasis: A forgotten disease, description and identification of species in 50 Colombian cases

• Published in: Biomédica Vol. 39; no. Supl. 2; pp. 58 - 65
• Main Authors: Muvdi-Arenas, Sandra, Ovalle-Bracho, Clemencia
• Format: Journal Article
• Language: English
• Published: Colombia Instituto Nacional de Salud 01.08.2019
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Child; Child, Preschool; Colombia - epidemiology; DNA, Protozoan - genetics; Female; Genes, Protozoan; HSP70 Heat-Shock Proteins - genetics; Humans; Leishmania; Leishmania braziliensis - classification; Leishmania braziliensis - genetics; Leishmania braziliensis - isolation & purification; Leishmania guyanensis - classification; Leishmania guyanensis - genetics; Leishmania guyanensis - isolation & purification; leishmaniasis, mucocutaneous; Leishmaniasis, Mucocutaneous - complications; Leishmaniasis, Mucocutaneous - epidemiology; Leishmaniasis, Mucocutaneous - parasitology; Leishmaniasis, Mucocutaneous - pathology; Male; Middle Aged; molecular typing; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Protozoan Proteins - genetics; Sequence Analysis, DNA; Skin - parasitology; Species Specificity; TROPICAL MEDICINE; Young Adult; Colombia; tipificación molecular; reacción en cadena de la polimerasa; Leishmania; leishmaniasis mucocutánea; leishmaniasis, mucocutaneous; molecular typing; polymerase chain reaction
• ISSN: 0120-4157; 2590-7379
• DOI: 10.7705/biomedica.v39i3.4347

Mucosal leishmaniasis has a progressive course and can cause deformity and even mutilation in the affected areas. It is endemic in the American continent and it is mainly caused by Leishmania (Viannia) braziliensis. To describe a series of mucosal leishmaniasis cases and the infectious Leishmania species. We included 50 patients with a clinical diagnosis of mucosal leishmaniasis and parasitological confirmation, and we described their clinical and laboratory results. We performed species typing by PCR-RFLP using the miniexon sequence and hsp70 genes; confirmation was done by sequencing. The median time of disease evolution was 2.9 years (range: 1 month to 16 years). The relevant clinical findings included mucosal infiltration (94%), cutaneous leishmaniasis scar (74%), total loss of the nasal septum (24%), nasal deformity (22%), and mucosal ulceration (38%). The symptoms reported included nasal obstruction (90%), epistaxis (72%), rhinorrhea (72%), dysphonia (28%), dysphagia (18%), and nasal pruritus (34%). The histopathological study revealed a pattern compatible with leishmaniasis in 86% of the biopsies, and amastigotes were identified in 14% of them. The Montenegro skin test was positive in 86% of patients, immunofluorescence in 84%, and culture in 8%. Leishmania (V.) braziliensis was identified in 88% of the samples, L. (V) panamensis in 8%, and L. (V.) guyanensis and L. (L.) amazonensis in 2% respectively. In this study, we found a severe nasal disease with destruction and deformity of the nasal septum in 25% of the cases, probably associated with late diagnosis. Leishmania (V.) braziliensis was the predominant species. We described a case of mucosal leishmaniasis in Colombia caused by L. (L.) amazonensis for the first time.