Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish (Cyclopterus lumpus L.)

The use of lumpfish (Cyclopterus lumpus) as a cleaner fish to fight sea lice infestation in farmed Atlantic salmon has become increasingly common. Still, tools to increase our knowledge about lumpfish biology are lacking. Here, we successfully established and characterized the first Lumpfish Gill ce...

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Published inCells (Basel, Switzerland) Vol. 10; no. 9; p. 2442
Main Authors Sindre, Hilde, Gjessing, Mona C., Fosse, Johanna Hol, Hermansen, Lene C., Böckerman, Inger, Amundsen, Marit M., Dahle, Maria K., Solhaug, Anita
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 16.09.2021
MDPI
Subjects
Aquaculture
Biology
Cell culture
cleaner fish
Cohabitation
Cyclopterus lumpus
Cytochrome P450
Disease
endothelial
Endothelial cells
epithelial
Farms
Hemorrhage
Hemorrhagic septicemia
Host-pathogen interactions
Infectious diseases
Infectious hematopoietic necrosis
lumpfish
Pathogens
respiration
Salmo salar
Salmon
Septicemia
Transmission electron microscopy
Trout
virus
Viruses
United States > US
Iceland
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Summary:The use of lumpfish (Cyclopterus lumpus) as a cleaner fish to fight sea lice infestation in farmed Atlantic salmon has become increasingly common. Still, tools to increase our knowledge about lumpfish biology are lacking. Here, we successfully established and characterized the first Lumpfish Gill cell line (LG-1). LG-1 are adherent, homogenous and have a flat, stretched-out and almost transparent appearance. Transmission electron microscopy revealed cellular protrusions and desmosome-like structures that, together with their ability to generate a transcellular epithelial/endothelial resistance, suggest an epithelial or endothelial cell type. Furthermore, the cells exert Cytochrome P450 1A activity. LG-1 supported the propagation of several viruses that may lead to severe infectious diseases with high mortalities in fish farming, including viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). Altogether, our data indicate that the LG-1 cell line originates from an epithelial or endothelial cell type and will be a valuable in vitro research tool to study gill cell function as well as host-pathogen interactions in lumpfish.
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ISSN:2073-4409
2073-4409
DOI:10.3390/cells10092442