Isolation techniques for anthocyanidin 3,5-diglucosides and their related chemicals using supramolecules technique, and two solid-phase extraction cartridges

• Published in: Journal of Chromatography A Vol. 1351; pp. 21 - 28
• Main Authors: Asada, Takayuki, Koi, Yoriko, Arakawa, Risa, Zhu, Fengxian, Sadaoka, Mayumi, Tamura, Hirotoshi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 18.07.2014; Elsevier
• Subjects: Anthocyanidin 3 ;5-diglucosides; Anthocyanins - chemistry; Biological and medical sciences; Cartridges; Chemical constitution; Chemistry - methods; Chromatography; Derivatives; Discovery DPA-6S and DSC-SCX; Extraction; Flavonoids; Fundamental and applied biological sciences. Psychology; Glucosides - chemistry; Glucosides - isolation & purification; Hydrolysis; Isolation technique; Perilla - chemistry; Pigments; Plant physiology and development; Purity; Solid Phase Extraction; Supramolecule; Supramolecule; Discovery DPA-6S and DSC-SCX; Isolation technique; Anthocyanidin 3 ;5-diglucosides; Anthocyanin; Solid phase extraction; Purification; Aldose; Glucoside; Flavonoid; Chemical enrichment; Acid hydrolysis; Polyphenol; Sample preparation; Supramolecular structure; Pigments; Isolation; Glycoside; Cation exchanger
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2014.05.039

Isolation techniques for anthocyanidin 3,5-diglucosides and their derivatives from three types of supramolecular pigments were newly developed using two SPE (Discovery DPA-6S and DSC-SCX) cartridges. Initially, malonylawobanin from commelinin supramolecular complex yielded 79.5% with 85.7% purity using DPA-6S, while DSC-SCX gave a rather lower yield (62.5%) of malonylawobanin with high purity (90.8%). Delphinidin 3,5-diglucoside and awobanin from acidic hydrolysis of commelinin were easily separated using DPA-6S. Secondary, artificial Perilla supramolecules (reconstructed from Perilla crude pigment, flavocommelin and Mg2+) result in a high purity of a malonylshisonin and shisonin mixture (96.1%) with DSC-SCX. Finally, succinylcyanin, which is a component of protocyanin, yielded 64.3% with 86.1% purity using DPA-6S and 51.8% with 74.1% purity using DSC-SCX. Moreover, cyanidin 3,5-diglucoside yielded 43.4% with 97.0% purity using DPA-6S after partial acidic hydrolysis of protocyanin.