Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis

In our previous study, human fibroblast growth factor 1 was successfully fused with oleosomes, energy-storing organelles of seeds, which are considered to be excellent “expression carriers” for substances with a convenient purification process. The present work aimed to explore the beneficial effect...

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Published inInternational journal of molecular sciences Vol. 23; no. 21; p. 13152
Main Authors Guo, Yongxin, Chu, Guodong, Cai, Weijia, Li, Yaying, Lan, Xinxin, Li, Jing, Du, Linna, Yang, Jing
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.11.2022
MDPI
Subjects
1-Phosphatidylinositol 3-kinase
AKT protein
Angiogenesis
Blood vessels
Cell proliferation
Chorioallantoic membrane
Collagen
Drug development
Endothelial cells
Fibroblast growth factor 1
Fibroblasts
Growth factors
human umbilical vein endothelial cells
oleosomes
Organelles
Physiology
proliferation
Proteins
Seeds
Sequence analysis
Skin
Transcriptomes
Umbilical vein
wound
Wound healing
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Summary:In our previous study, human fibroblast growth factor 1 was successfully fused with oleosomes, energy-storing organelles of seeds, which are considered to be excellent “expression carriers” for substances with a convenient purification process. The present work aimed to explore the beneficial effects of oleosomes fused with human fibroblast growth factor 1 (OLAF) on wound healing. The data showed marked improvements in terms of the angiogenesis, vascular integrity, collagen and inflammation on the wound sites of rats with a full-thickness skin defect. Moreover, the positive role of OLAF in promoting angiogenesis and its possible pathways were clarified in vivo and in vitro. The results showed that the number, length and branches of the blood vessels of the chick embryo chorioallantoic membrane were markedly increased after OLAF treatment. Meanwhile, the in vitro results also revealed that 100 ng/mL OLAF exhibited a promoting effect on the proliferation, migration and tube formation of human umbilical vein endothelial cells. In addition, the potential of OLAF to improve wound angiogenesis was demonstrated to be associated with an up-regulated PI3K/Akt pathway by transcriptome sequencing analysis and the introduction of a PI3K/Akt pathway inhibitor (LY294002). These findings suggest that OLAF has many prospects in the development of drugs for wound healing.
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These authors contributed equally to this manuscript.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232113152