An improved immunolabeling method for microtubular cytoskeleton in poplar (Populus nigra L.) free nuclear endosperm

We investigated immunocytochemical staining of microtubular cytoskeleton of free nuclear endosperm, a tissue which is particularly difficult to fix. This tissue requires fixation for 45 hr to preserve the integrity of the microtubular network after paraformaldehyde based fixation. Low glutaraldehyde...

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Bibliographic Details
Published inBiotechnic & histochemistry Vol. 70; no. 5; p. 252
Main Authors Xu, X, Souvré, A, Granier, C, Petitprez, M
Format Journal Article
LanguageEnglish
Published England 1995
Subjects
aldehidos
aldehyde
aldehydes
Cell Nucleus - chemistry
cell structure
Cytoskeleton - chemistry
endosperm
endosperma
endosperme
estructura celular
Ethanol
Fixatives
formaldehidos
Formaldehyde
glicoproteinas
Glutaral
glycoproteine
glycoproteins
Immunohistochemistry
immunologie
immunology
inmunologia
Microtubules - chemistry
noyau cellulaire
nucleo
nucleus
Polymers
populus nigra
structure cellulaire
Time Factors
Tissue Embedding - methods
Tissue Fixation - methods
Trees
Tubulin - analysis
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Summary:We investigated immunocytochemical staining of microtubular cytoskeleton of free nuclear endosperm, a tissue which is particularly difficult to fix. This tissue requires fixation for 45 hr to preserve the integrity of the microtubular network after paraformaldehyde based fixation. Low glutaraldehyde concentration in the fixative and the ethanol dehydration retains beta-tubulin antigenicity and the former improves preservation of tissue structure. An ethanol-free embedding method is recommended for immunocytochemical studies of ethanol sensitive target proteins.
Bibliography:F50
F
ISSN:1052-0295
1473-7760
DOI:10.3109/10520299509108202