A Combinatorial Code for the Interaction of α-Synuclein with Membranes

Considerable genetic and pathological evidence has implicated the small, soluble protein α-synuclein in the pathogenesis of familial and sporadic forms of Parkinsons disease (PD). However, the precise role of α-synuclein in the disease process as well as its normal function remain poorly understood....

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Published inThe Journal of biological chemistry Vol. 280; no. 36; pp. 31664 - 31672
Main Authors Kubo, Shin-ichiro, Nemani, Venu M., Chalkley, Robert J., Anthony, Malcolm D., Hattori, Nobutaka, Mizuno, Yoshikuni, Edwards, Robert H., Fortin, Doris L.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 09.09.2005
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Summary:Considerable genetic and pathological evidence has implicated the small, soluble protein α-synuclein in the pathogenesis of familial and sporadic forms of Parkinsons disease (PD). However, the precise role of α-synuclein in the disease process as well as its normal function remain poorly understood. We recently found that an interaction with lipid rafts is crucial for the normal, pre-synaptic localization of α-synuclein. To understand how α-synuclein interacts with lipid rafts, we have now developed an in vitro binding assay to rafts purified from native membranes. Recapitulating the specificity observed in vivo, recombinant wild type but not PD-associated A30P mutant α-synuclein binds to lipid rafts isolated from cultured cells and purified synaptic vesicles. Proteolytic digestion of the rafts does not disrupt the binding of α-synuclein, indicating an interaction with lipid rather than protein components of these membranes. We have also found that α-synuclein binds directly to artificial membranes whose lipid composition mimics that of lipid rafts. The binding of α-synuclein to these raft-like liposomes requires acidic phospholipids, with a preference for phosphatidylserine (PS). Interestingly, a variety of synthetic PS with defined acyl chains do not support binding when used individually. Rather, the interaction with α-synuclein requires a combination of PS with oleic (18:1) and polyunsaturated (either 20:4 or 22:6) fatty acyl chains, suggesting a role for phase separation within the membrane. Furthermore, α-synuclein binds with higher affinity to artificial membranes with the PS head group on the polyunsaturated fatty acyl chain rather than on the oleoyl side chain, indicating a stringent combinatorial code for the interaction of α-synuclein with membranes.
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ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M504894200