The effect of elevated progesterone levels before oocyte retrieval in women undergoing ovarian stimulation for IVF treatment on the genomic profile of peri-implantation endometrium

• Published in: Journal of reproductive immunology Vol. 121; pp. 17 - 25
• Main Authors: Liu, Liu, Huang, Jin, Li, Tin Chiu, Hong, Xu Tao, Laird, Susan, Dai, Yong Dong, Tong, Xiao Mei, Zhu, Hai Yan, Zhang, Songying
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.06.2017
• Subjects: Adult; Cytotoxicity, Immunologic; Embryo Implantation; Endometrium; Endometrium - physiology; Female; Fertilization in Vitro; Gene Expression Profiling; Genome - genetics; Humans; IVF; Killer Cells, Natural - immunology; Obstetrics and Gynecology; Oocyte Retrieval; Ovulation Induction; Peri-implantation; Pregnancy; Progesterone; Progesterone - metabolism; Prospective Studies; uNK cell; IVF; Progesterone; Peri-implantation; uNK cell; Endometrium
• ISSN: 0165-0378; 1872-7603; 1872-7603
• DOI: 10.1016/j.jri.2017.05.001

•The impact of high Progesterone on genomic profile of the endometrium at the time of implantation was examined.•It confirms the importance of obtaining Progesterone measurement on two consecutive days prior to oocyte recovery.•The major pathway affected is NK cell mediated cytotoxicity. To evaluate the effects of high progesterone prior to oocyte retrieval on the genomic profile of peri-implantation endometrium, we conducted this single-center, prospective cohort study. Depending on whether or not the progesterone level on the day of hCG administration and the day after hCG administration were elevated, a total of 20 women undergoing IVF treatment who did not have fresh embryo transfer were included: Group 1 refers to subjects with normal progesterone level on both days; Group 2 refers to subjects with normal progesterone level on the day of hCG administration and high progesterone level on the day after hCG administration; Group 3 refers to subjects with high progesterone level on the day of hCG administration and normal progesterone level on the day after hCG administration; Group 4 refers to subjects with high progesterone level on both days. Five subjects were included in each group. Endometrial samples were obtained 7days after hCG administration. We found that high progesterone level prior to oocyte retrieval predominantly affected components of the NK cell mediated cytotoxicity pathway in the endometrium and that significant differences were only seen when progesterone measurements on both the day of and day after hCG administration were considered together.