Determination of inorganic anions in saliva by capillary isotachophoresis

• Published in: Talanta (Oxford) Vol. 59; no. 4; pp. 643 - 649
• Main Authors: SADECKA, Jana, POLONSKY, Jozef
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10.03.2003; Oxford Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Anions; Biological and medical sciences; Capillary isotachophoresis; Fundamental and applied biological sciences. Psychology; Inorganic compounds; Other biological molecules; Saliva; Saliva; Anions; Capillary isotachophoresis; Capillary column; Biological fluid; Iodides; Nitrites; Nitrates; Isotachophoresis; Inorganic anion; Analysis method; Simultaneous measurement; Thiocyanates; Quantitative analysis
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/S0039-9140(02)00467-8

Nitrite, nitrate, iodide and thiocyanate have been quantified in non-smoker and smoker saliva by capillary isotachophoresis (CITP). Hydrochloric acid (10 mmol l −1) adjusted with histidine to pH 6.0 plus 6% poly(vinylpyrrolidone) was used as the leading electrolyte (LE) and 5 mmol l −1 acetic acid as the terminating electrolyte (TE). Linearity was observed from 0.005 to 0.500 mmol l −1 with a coefficient of determination ( r 2) of 0.999. The separation of anions was achieved in less than 19 min. The minimal sample pretreatment and relatively low running cost make isotachophoresis good alternative to existing methods.