A highly conserved Brassica gene with homology to the S-locus-specific glycoprotein structural gene

• Published in: The Plant cell Vol. 1; no. 2; pp. 249 - 258
• Main Authors: Lalonde, Beth A., Nasrallah, Mikhail E., Dwyer, Kathleen G., Chen, Che-Hong, Barlow, Bicka, Nasrallah, June B.
• Format: Journal Article
• Language: English
• Published: United States American Society of Plant Physiologists 01.02.1989
• Subjects: Amino Acid Sequence; Amino acids; analytical methods; arn mensajero; arn messager; Base Sequence; Brassica - genetics; brassica oleracea; brassica oleracea italica; clonacion; clonage; cloning; Cloning, Molecular; Complementary DNA; croisement; crossbreeding; cruciferae; cruzamiento; DNA; expresion genica; expression des genes; Flower stigma; forecasting; Gels; gene; gene expression; Gene Expression Regulation; Genes; Genes, Plant; Genetic Linkage; Genomics; Genotype; gineceo; glicoproteinas; glycoproteine; Glycoproteins; Glycoproteins - genetics; gynecee; gynoecium; messenger rna; Molecular Sequence Data; nucleotide; nucleotides; nucleotidos; Plant Proteins - genetics; Plants; radiografia; radiographie; radiography; Reproduction - genetics; RNA; segregacion; segregation; Sequence Homology, Nucleic Acid; technique analytique; technique de prevision; technique des traceurs; tecnicas analiticas; tecnicas de prediccion; tecnicas de trazadores; tracer techniques
• ISSN: 1040-4651; 1532-298X
• DOI: 10.1105/tpc.1.2.249

The S-locus-specific glycoprotein of Brassica and the gene encoding it (the SLG gene) are thought to be involved in determining self-incompatibility phenotype in this genus. It has been shown that the Brassica genome contains multiple SLG-related sequences. We report here the cloning and characterization of a Brassica oleracea gene, SLR1, which corresponds to one of these SLG-related sequences. Like the SLG gene, SLR1 is developmentally regulated. It is maximally expressed in the papillar cells of the stigma at the same stage of flower development as the onset of the incompatibility response. Unlike SLG, the SLR1 genes isolated from different S-allele homozygotes are highly conserved, and this gene, which appears to be ubiquitous in crucifers, is expressed in self-compatible strains as well as self-incompatible strains. Most importantly, we show that the SLR1 gene is not linked to the S-locus and therefore cannot be a determinant of S-allele specificity in Brassica.