Synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supports

The effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe /SiO was modified with 3-aminopropyl-triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h were...

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Bibliographic Details
Published inGreen processing and synthesis Vol. 6; no. 4; pp. 377 - 384
Main Authors Vargas, Annie Y., Rojas, Hugo A., Romanelli, Gustavo P., Martínez, José J.
Format Journal Article
LanguageEnglish
Published Berlin De Gruyter 28.08.2017
Walter de Gruyter GmbH
Subjects
Adsorption
Biginelli/Hantzsch reaction
Chemical synthesis
Covalence
Enzymatic activity
Glutaraldehyde
Immobilization
Magnetic properties
magnetic supports
Urease
urease immobilized
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Summary:The effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe /SiO was modified with 3-aminopropyl-triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h were sufficient for the physical adsorption, while 24 h were required for covalent bonding. The Biginelli and Hantzsch reactions were used to evaluate urease application in multicomponent reactions (MCRs). The synthesis of 1,4-dihydropyrimidines was successfully performed using immobilized urease favoring the Hantzsch product. The magnetic properties of the supports allow easy separation, and the urease immobilized by both methods improved the enzymatic activity compared to that of free urease.
ISSN:2191-9542
2191-9550
DOI:10.1515/gps-2016-0143