A method for single-neuron chronic recording from the retina in awake mice

The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo rec...

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Published inScience (American Association for the Advancement of Science) Vol. 360; no. 6396; pp. 1447 - 1451
Main Authors Hong, Guosong, Fu, Tian-Ming, Qiao, Mu, Viveros, Robert D, Yang, Xiao, Zhou, Tao, Lee, Jung Min, Park, Hong-Gyu, Sanes, Joshua R, Lieber, Charles M
Format Journal Article
LanguageEnglish
Published United States The American Association for the Advancement of Science 29.06.2018
Subjects
Animals
Brain
Circadian Rhythm
Circadian rhythms
Circuits
Electrodes, Implanted
Electronics
Electrophysiology
Eye
Finite element method
In vivo methods and tests
Information processing
Information systems
Injection
Mice
Microelectrodes
Neural networks
Neural Pathways - physiology
Orientation
Photic Stimulation
Retina
Retinal ganglion cells
Retinal Ganglion Cells - physiology
Single-Cell Analysis - methods
Surgical implants
Visual stimuli
Visual system
Wakefulness
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Summary:The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.
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These authors contributed equally to this work.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.aas9160