Immunohistochemical detection of SWC3, CD2, CD3, CD4 and CD8 antigens in paraformaldehyde fixed and paraffin embedded porcine lymphoid tissue

• Published in: Veterinary immunology and immunopathology Vol. 94; no. 3; pp. 123 - 132
• Main Authors: Tingstedt, Jens-Erik, Tornehave, Ditte, Lind, Peter, Nielsen, Jens
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.08.2003
• Subjects: Animals; Antibodies, Monoclonal; Antigen retrieval; Antigens, CD - analysis; Antigens, CD - metabolism; CD antigens; CD2 antigen; CD3 antigen; CD4 antigen; CD8 antigen; Formaldehyde; Immunohistochemistry; Immunohistochemistry - methods; Immunohistochemistry - veterinary; Leukocytes; Lymph Nodes - cytology; Lymph Nodes - immunology; Paraffin Embedding - methods; Paraffin Embedding - veterinary; Pig; Specific Pathogen-Free Organisms; SWC3 antigen; Swine - immunology; Swine - metabolism; Tissue Fixation - methods; Tissue Fixation - veterinary; Tyramide signal amplification; CD antigens; Immunohistochemistry; Leukocytes; Tyramide signal amplification; Antigen retrieval; Pig
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/S0165-2427(03)00096-5

Identification of the different cell types of the immune system is important for in situ studies on the pathogenesis of infectious diseases in various animals, including the pig. Unfortunately, many monoclonal anti-leukocyte antibodies are only useful for staining frozen tissue sections with inherent poor tissue morphology, and are not readily adapted to formaldehyde fixed and paraffin embedded tissue with well preserved morphology. Seven well characterised monoclonal antibodies against porcine leukocyte antigens were tested on neutral buffered paraformaldehyde fixed and paraffin embedded porcine tissue sections using the highly sensitive tyramide signal amplification system. Combining this method with different antigen retrieval techniques enabled us to detect CD2, CD3, CD4, CD8 and SWC3 antigen expressing cells in porcine lymphoid tissue. Thus, we describe herein methods for the detection of several major cell types of the porcine immune system in fixed tissue with optimal preservation of histological details.