In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus

The thermophilic, endospore-forming genus of has historically been associated with spoilage of canned food. However, in recent years it has become the subject of much attention due its biotechnological potential in areas such as enzyme and biofuel applications. One aspect of this genus that has not...

Full description

Saved in:
Bibliographic Details
Published inFrontiers in microbiology Vol. 9; p. 2116
Main Authors Egan, Kevin, Field, Des, Ross, R. Paul, Cotter, Paul D., Hill, Colin
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 20.09.2018
Subjects
antimicrobial
bacteriocin
bioinformatics
Geobacillus
in silico screen
Microbiology
antimicrobial
Geobacillus
in silico screen
bacteriocin
bioinformatics
Online AccessGet full text

Cover

More Information
Summary:The thermophilic, endospore-forming genus of has historically been associated with spoilage of canned food. However, in recent years it has become the subject of much attention due its biotechnological potential in areas such as enzyme and biofuel applications. One aspect of this genus that has not been fully explored or realized is its use as a source of novel forms of the ribosomally synthesized antimicrobial peptides known as bacteriocins. To date only two bacteriocins have been fully characterized within this genus, i.e., Geobacillin I and II, with only a small number of others partially characterized. Here we bioinformatically investigate the potential of this genus as a source of novel bacteriocins through the use of the screening software BAGEL3, which scans publically available genomes for potential bacteriocin gene clusters. In this study we examined the association of bacteriocin gene presence with niche and phylogenetic position within the genus. We also identified a number of candidates from multiple bacteriocin classes which may be promising antimicrobial candidates when investigated in future studies.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Reviewed by: Gustavo Hernandez Guzman, Universidad de Guanajuato, Mexico; Jin Zhong, State Key Laboratory of Microbial Resources, Institute of Microbiology (CAS), China; Agustino Martínez Antonio, Unidad Irapuato (CINVESTAV), Mexico
Edited by: José E. Barboza-Corona, Universidad de Guanajuato, Mexico
This article was submitted to Microbiotechnology, Ecotoxicology and Bioremediation, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2018.02116