Liquid chromatographic method for determination of piperine in rat plasma: application to pharmacokinetics

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 776; no. 2; pp. 245 - 249
• Main Authors: Bajad, Sunil, Singla, A.K, Bedi, K.L
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.09.2002
• Subjects: Alkaloids; Animals; Benzodioxoles; Body fluids; Chromatography, High Pressure Liquid - methods; Correlation methods; Extraction; Pharmacokinetics; Piperidines - blood; Piperidines - pharmacokinetics; Piperine; Polyunsaturated Alkamides; Rats; Spectrophotometry, Ultraviolet; Toxicity; Ultraviolet detectors; Piperine
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/S1570-0232(02)00352-5

Piperine, a major alkaloid of Piper longum and Piper nigrum has been reported to have several pharmacological/toxicological effects. Though a number of methods for analysis of this omnipresent food component in pepper fruits are available, its analysis in body fluids has been largely neglected. A high-performance liquid chromatography method for the analysis of piperine in rat plasma is presented in this communication. Analysis was performed using a Symmetry ® C 18 column (250×4.6 mm) by isocratic elution with 25 m M KH 2PO 4 (pH 4.5)–acetonitrile (35:65) and UV detection at 340 nm. The calibration plot was linear over the range studied (2–2000 ng) with correlation coefficient of 0.9984. Limit of detection and limit of quantitation were 1 ng/ml and 3 ng/ml, respectively. Good overall recovery (85.5±6%) was obtained with 4 ml ethyl acetate and extraction time of 3 min. Intra- and inter-assay coefficient of variation was found to be less than 7.5%. Plasma concentration–time profile of piperine in a conscious rat implanted with jugular vein cannula was obtained using this method. The method is simple, sensitive and reproducible.