A safe, efficient and environment friendly biosynthesis of silver nanoparticles using Leucaena leucocephala seed extract and its antioxidant, antimicrobial, antifungal activities and potential in sensing

• Published in: Green processing and synthesis Vol. 6; no. 5; pp. 449 - 459
• Main Authors: Kumar, Rajeev, Sharma, Priyanka, Bamal, Aditi, Negi, Sushma, Chaudhary, Savita
• Format: Journal Article
• Language: English
• Published: Berlin De Gruyter 26.09.2017; Walter de Gruyter GmbH
• Subjects: Antifungal activity; Antiinfectives and antibacterials; Antioxidants; bioreduction; Biosynthesis; DPPH; E coli; echinodontium taxodii; Energy transmission; escherichia coli; Flavonoids; Fourier transforms; Fungi; Fungicides; Infrared analysis; Infrared spectrophotometers; Infrared spectroscopy; Leucaena leucocephala; Light scattering; Nanoparticles; phlebiopsis gigantea; Phytochemicals; Qualitative analysis; Scanning electron microscopy; Seeds; sensing; Silver; silver nanoparticles; Silver nitrate; Spectrophotometry; Spectrum analysis; staphylococcus aureus; Sterols; Terpenes; Thermogravimetric analysis; Transmission electron microscopy; Ultraviolet radiation; Ultraviolet spectroscopy; X-ray diffraction
• ISSN: 2191-9542; 2191-9550
• DOI: 10.1515/gps-2016-0146

One step green synthesis of silver nanoparticles (AgNPs) from silver nitrate (AgNO ) using seeds extract as the reducing agent at room temperature was performed. The bioreduced NPs were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) coupled with energy dispersive spectrophotometry, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA) techniques. Qualitative information of major components in the seed extract was obtained through its phytochemical screening. The phytochemical data of revealed the presence of terpenes, flavonoids, coumarins and sterols. The reaction was optimized for AgNO , extract concentration and time duration for the reaction. The obtained NPs showed a characteristic UV peak of AgNPs at 420 nm. TEM and SEM images showed the spherical shaped NPs over which the extract coating was very prominent. The binding of seeds extract onto NPs was tested using FTIR and TGA. The antifungal activity of the as-synthesized NPs against two fungal species, namely and , was studied. The antimicrobial effect of the as-synthesized NPs was ascertained against and . The antioxidant potential of the AgNPs was tested with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Also, the sensitivity of the NPs towards Fe ions was tested in aqueous media.