Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications

• Published in: Journal of Chromatography A Vol. 1372; pp. 1 - 17
• Main Authors: Huang, Junfeng, Wang, Fangjun, Ye, Mingliang, Zou, Hanfa
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 12.12.2014; Elsevier
• Subjects: Acetylation; Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Enrichment; Exact sciences and technology; fractionation; Fundamental and applied biological sciences. Psychology; General aspects, investigation methods; glycosylation; Government regulations; Liquid chromatography; Mass spectrometry; methylation; Multi-dimensional liquid chromatography separation; Other chromatographic methods; oxidation; peptides; phosphorylation; Post-translational modifications; Protein glycosylation; Protein phosphorylation; Proteins; proteome; Proteomics; Separation; Strategy; ubiquitination; Enrichment; Post-translational modifications; Protein glycosylation; Protein phosphorylation; Mass spectrometry; Multi-dimensional liquid chromatography separation; Trace analysis; Multidimensional chromatography; Tandem mass spectrometry; Phosphorylation; Chemical analysis; Peptides; Coupled method; HPLC chromatography; Glycosylation; Review; Protein; Chemical enrichment; Posttranslational modification; Analysis method; Peptide fragment; Proteomics; Large scale
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2014.10.107

•Major protein post-translational modifications (PTMs) and their enrichment strategies.•The challenges encountered in the current PTMs enrichment methods.•Separation strategies of PTM peptides for large scale PTMs analysis. Comprehensive analysis of the post-translational modifications (PTMs) on proteins at proteome level is crucial to elucidate the regulatory mechanisms of various biological processes. In the past decades, thanks to the development of specific PTM enrichment techniques and efficient multidimensional liquid chromatography (LC) separation strategy, the identification of protein PTMs have made tremendous progress. A huge number of modification sites for some major protein PTMs have been identified by proteomics analysis. In this review, we first introduced the recent progresses of PTM enrichment methods for the analysis of several major PTMs including phosphorylation, glycosylation, ubiquitination, acetylation, methylation, and oxidation/reduction status. We then briefly summarized the challenges for PTM enrichment. Finally, we introduced the fractionation and separation techniques for efficient separation of PTM peptides in large-scale PTM analysis.