Expression of a fungal sesquiterpene cyclase gene in transgenic tobacco

• Published in: Plant physiology (Bethesda) Vol. 97; no. 1; pp. 460 - 462
• Main Authors: Hohn, T.M. (National Center for Agricultural Utilization Research, ARS, USDA, Peoria, IL), Ohlrogge, J.B
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.09.1991
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; Biosynthesis; Cell culture techniques; Communications; Enzymes; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; FUSARIUM; Gene expression regulation; genes; Leaves; LIGASAS; LIGASE; NICOTIANA TABACUM; Plant physiology and development; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; Sesquiterpenes; SESQUITERPENOIDE; SESQUITERPENOS; Terpenoids; Tissue cultures, protoplasts; transformation; Transgenic plants; Enzyme; Fusarium sporotrichioides; Gene expression; Nicotiana tabacum; Fungi; Dicotyledones; Angiospermae; Industrial crop; Spermatophyta; Transgenic plant; Fungi Imperfecti; Solanaceae; Thallophyta
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.97.1.460

The complete coding sequence for the trichodiene synthase gene from Fusarium sporotrichioides was introduced into tobacco (Nicotiana tabacum) under the regulation of the cauliflower mosaic virus 35S promoter. Expression of trichodiene synthase was demonstrated in the leaves of transformed plants. Leaf homogenates incubated with [3H]farnesyl pyrophosphate produced trichodiene as a major product. Trichodiene was detected in the leaves of a transformed plant at a level of 5 to 10 nanograms per gram fresh weight. The introduction of a fungal sesquiterpene cyclase gene into tobacco has resulted in the expression of an active enzyme and the accumulation of low levels of its sesquiterpenoid product