In vivo detection of single cells by MRI
The use of high‐relaxivity, intracellular contrast agents has enabled MRI monitoring of cell migration through and homing to various tissues, such as brain, spinal cord, heart, and muscle. Here it is shown that MRI can detect single cells in vivo, homing to tissue, following cell labeling and transp...
Saved in:
Published in | Magnetic resonance in medicine Vol. 55; no. 2; pp. 242 - 249 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.02.2006
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The use of high‐relaxivity, intracellular contrast agents has enabled MRI monitoring of cell migration through and homing to various tissues, such as brain, spinal cord, heart, and muscle. Here it is shown that MRI can detect single cells in vivo, homing to tissue, following cell labeling and transplantation. Primary mouse hepatocytes were double‐labeled with green fluorescent 1.63‐μm iron oxide particles and red fluorescent endosomal labeling dye, and injected into the spleens of recipient mice. This is a common hepatocyte transplantation paradigm in rodents whereby hepatocytes migrate from the spleen to the liver as single cells. One month later the animals underwent in vivo MRI and punctuated, dark contrast regions were detected scattered through the livers. MRI of perfused, fixed samples and labeled hepatocyte phantoms in combination with histological evaluation confirmed the presence of dispersed single hepatocytes grafted into the livers. Appropriate controls were used to determine whether the observed contrast could have been due to dead cells or free particles, and the results confirmed that the contrast was due to disperse, single cells. Detecting single cells in vivo opens the door to a number of experiments, such as monitoring rare cellular events, assessing the kinetics of stem cell homing, and achieving early detection of metastases. Magn Reson Med, 2006. Published 2006 Wiley‐Liss, Inc. |
---|---|
Bibliography: | ArticleID:MRM20718 ark:/67375/WNG-B8NVKPVD-H This article is a US Government work and, as such, is in the public domain in the United States of America. 2005 ISMRM Young Investigator W.S. Moore Award Finalist istex:AD2C94C8F85431CDC810011D35CD718F2FB263D8 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0740-3194 1522-2594 |
DOI: | 10.1002/mrm.20718 |